ID A0A1Y4DJU0_9ACTN Unreviewed; 918 AA.
AC A0A1Y4DJU0;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=B5F74_09175 {ECO:0000313|EMBL:OUO58952.1};
OS Collinsella sp. An271.
OC Bacteria; Actinomycetota; Coriobacteriia; Coriobacteriales;
OC Coriobacteriaceae; Collinsella.
OX NCBI_TaxID=1965616 {ECO:0000313|EMBL:OUO58952.1, ECO:0000313|Proteomes:UP000195889};
RN [1] {ECO:0000313|Proteomes:UP000195889}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=An271 {ECO:0000313|Proteomes:UP000195889};
RA Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA Rychlik I.;
RT "Function of individual gut microbiota members based on whole genome
RT sequencing of pure cultures obtained from chicken caecum.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUO58952.1}.
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DR EMBL; NFJE01000010; OUO58952.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y4DJU0; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000195889; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000195889};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 5..266
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 676..882
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 918 AA; 100001 MW; C5840AEBF25F24B7 CRC64;
MPDTRRTFAV IDGNSLMHRA FHAVPPTMNA PDGRPTNAIF GFLNMFLKMI DAFHPDGVAV
AFDRGKPRVR MEMLPQYKAQ RPPMDPDLRQ QFPMIKELLS KLSVPILEAE GWEGDDILGT
LARTGEAAGC DMYLITGDRD MYQLSTEHVK IVGTKKGLSD VQIMTPESVE DLYHGITPEL
VPDFYGLKGD TSDNIPGVPG IGPKKASALI AQYGSLDEVI AHADEVKGKM GENLRAHIDD
ALLSRKVATI QTNAPVELDF EHTAFPAYDA SSVADALGEL GINAMQNRFL ALIGGEGAPE
AAASTLDIPD ALHAYAATGE DVEQAARELA RAIEAGEWIA AVLDDDREAG ALFGLTHTLW
VAAPDALLAF EEPDGGACDL PKVDGYDFSH GLIAGVLLRL FGEGKVVSPD VKALIHELAP
ADSSVPAHID ALAIDPDRIF DTVVAAYLLD SARSSFDDGY IADAYLNVQL PAAAGEDGAA
EGAVPAAARS AVLARTALMP LAESLDRQGA ARVFTQIEMP LIPVLADMER TGMRLDPERL
SEMSDKLVGQ IDELAGRIRA LAGDDSFNIA SPMQLSHILF DVMGLPTKGL KKTQRGYYST
NAKVLDDLAR DHEIVRLILE WREKTKIKST YLDTLGQQRR GDGRVHTTYN QTITATGRLS
SSNPNLQNIP TRSELGHTVK TAFAAGEGAV FLAVDYSQIE LRLLAHLSED EHLVRAFNEG
EDFHAETAAR VFGVPVEEVT PQLRSRAKAV NFGIVYGQQA YGLSQSLDIP MAEAREMIEA
YFRAYPGVRT YLDRTVAEAK RTGYAETMFG RRRPIPELKM RNPAQRSFGE RTAMNHPMQG
SAADIIKIAM ARVARRLHDE GFAARMILQV HDELDFECPV DEVERLTAMV VEEMEGVVKL
RVPLIAEAST GVTWADAK
//
