ID A0A1Y4FXU3_9ACTN Unreviewed; 129 AA.
AC A0A1Y4FXU3;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 13-SEP-2023, entry version 26.
DE RecName: Full=6-carboxy-5,6,7,8-tetrahydropterin synthase {ECO:0000256|ARBA:ARBA00018141, ECO:0000256|PIRNR:PIRNR006113};
DE EC=4.-.-.- {ECO:0000256|PIRNR:PIRNR006113};
GN Name=queD {ECO:0000313|EMBL:ROT88097.1};
GN ORFNames=DMP12_13390 {ECO:0000313|EMBL:ROT88097.1}, GKG38_13680
GN {ECO:0000313|EMBL:MSA96085.1}, GO738_13520
GN {ECO:0000313|EMBL:MVN16344.1};
OS Gordonibacter urolithinfaciens.
OC Bacteria; Actinomycetota; Coriobacteriia; Eggerthellales; Eggerthellaceae;
OC Gordonibacter.
OX NCBI_TaxID=1335613 {ECO:0000313|EMBL:ROT88097.1, ECO:0000313|Proteomes:UP000285258};
RN [1] {ECO:0000313|Proteomes:UP000285258}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 27213 {ECO:0000313|Proteomes:UP000285258};
RA Danylec N., Stoll D.A., Doetsch A., Huch M.;
RT "Genome Sequencing of selected type strains of the family
RT Eggerthellaceae.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ROT88097.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 27213 {ECO:0000313|EMBL:ROT88097.1};
RX PubMed=31210629; DOI=.1099/ijsem.0.003537;
RA Danylec N., Stoll D.A., Huch M.;
RT "Gordonibacter faecihominis is a later heterotypic synonym of Gordonibacter
RT urolithinfaciens.";
RL Int. J. Syst. Evol. Microbiol. 69:2527-2532(2019).
RN [3] {ECO:0000313|EMBL:ROT88097.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 27213 {ECO:0000313|EMBL:ROT88097.1};
RX PubMed=30643901;
RA Danylec N., Stoll D.A., Dotsch A., Huch M.;
RT "Draft Genome Sequences of Type Strains of Gordonibacter faecihominis,
RT Paraeggerthella hongkongensis, Parvibacter caecicola,Slackia equolifaciens,
RT Slackia faecicanis, and Slackia isoflavoniconvertens.";
RL Microbiol. Resour. Announc. 8:e01532-18(2019).
RN [4] {ECO:0000313|EMBL:MSA96085.1, ECO:0000313|Proteomes:UP000462865}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BIOML-A1 {ECO:0000313|EMBL:MSA96085.1,
RC ECO:0000313|Proteomes:UP000462865};
RX PubMed=31477907; DOI=.1038/s41591-019-0559-3;
RA Poyet M., Groussin M., Gibbons S.M., Avila-Pacheco J., Jiang X.,
RA Kearney S.M., Perrotta A.R., Berdy B., Zhao S., Lieberman T.D.,
RA Swanson P.K., Smith M., Roesemann S., Alexander J.E., Rich S.A., Livny J.,
RA Vlamakis H., Clish C., Bullock K., Deik A., Scott J., Pierce K.A.,
RA Xavier R.J., Alm E.J.;
RT "A library of human gut bacterial isolates paired with longitudinal
RT multiomics data enables mechanistic microbiome research.";
RL Nat. Med. 25:1442-1452(2019).
RN [5] {ECO:0000313|EMBL:MVN16344.1, ECO:0000313|Proteomes:UP000468327}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ResAG-59 {ECO:0000313|EMBL:MVN16344.1,
RC ECO:0000313|Proteomes:UP000468327};
RA Stoll D.A., Danylec N., Franz C.M.A.P., Huch M.;
RT "Whole genome shotgun sequencing (WGS) data from Adlercreutzia
RT equolifaciens ResAG-91, Eggerthella lenta MRI-F36, MRI-F37, MRI-F40, ResAG-
RT 49, ResAG-88, ResAG-121, ResAG-145, and Gordonibacter sp. ResAG-5, ResAG-
RT 26, ResAG-43, ResAG-50, ResAG-59.";
RL Submitted (NOV-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7,8-dihydroneopterin 3'-triphosphate + H2O = 6-
CC carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) +
CC triphosphate; Xref=Rhea:RHEA:27966, ChEBI:CHEBI:15343,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18036,
CC ChEBI:CHEBI:58462, ChEBI:CHEBI:61032; EC=4.1.2.50;
CC Evidence={ECO:0000256|ARBA:ARBA00001293,
CC ECO:0000256|PIRNR:PIRNR006113};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRNR:PIRNR006113,
CC ECO:0000256|PIRSR:PIRSR006113-2};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRNR:PIRNR006113,
CC ECO:0000256|PIRSR:PIRSR006113-2};
CC -!- PATHWAY: Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005061, ECO:0000256|PIRNR:PIRNR006113}.
CC -!- SIMILARITY: Belongs to the PTPS family. QueD subfamily.
CC {ECO:0000256|ARBA:ARBA00008900, ECO:0000256|PIRNR:PIRNR006113}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ROT88097.1}.
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DR EMBL; WKZA01000099; MSA96085.1; -; Genomic_DNA.
DR EMBL; WPOC01000029; MVN16344.1; -; Genomic_DNA.
DR EMBL; QIBW01000024; ROT88097.1; -; Genomic_DNA.
DR UniPathway; UPA00391; -.
DR Proteomes; UP000285258; Unassembled WGS sequence.
DR Proteomes; UP000462865; Unassembled WGS sequence.
DR Proteomes; UP000468327; Unassembled WGS sequence.
DR GO; GO:0070497; F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.479.10; 6-pyruvoyl tetrahydropterin synthase/QueD; 1.
DR InterPro; IPR007115; 6-PTP_synth/QueD.
DR InterPro; IPR038418; 6-PTP_synth/QueD_sf.
DR NCBIfam; TIGR03367; queuosine_QueD; 1.
DR PANTHER; PTHR12589:SF7; 6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE; 1.
DR PANTHER; PTHR12589; PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE; 1.
DR Pfam; PF01242; PTPS; 1.
DR PIRSF; PIRSF006113; PTP_synth; 1.
DR SUPFAM; SSF55620; Tetrahydrobiopterin biosynthesis enzymes-like; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR006113};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR006113};
KW Queuosine biosynthesis {ECO:0000256|PIRNR:PIRNR006113};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRNR:PIRNR006113}.
FT ACT_SITE 31
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006113-1"
FT ACT_SITE 75
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR006113-1"
FT ACT_SITE 119
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR006113-1"
FT BINDING 22
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR006113-2"
FT BINDING 35
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR006113-2"
FT BINDING 37
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR006113-2"
SQ SEQUENCE 129 AA; 14639 MW; 02FD3FF47579E950 CRC64;
MHLHVGGGHY ELTVKDHFDA AHALPGYDGP CQYLHGHTWE VEATIAGQHL DEVGMLYDFK
DVKRDLHGVL ENFDHRCLND VPPFDEINPT AEHLARVVFY ELEKTFPAHI SLVEVAVWES
PAAKVVYRP
//
