ID   A0A1Y4IFM5_9FIRM        Unreviewed;       287 AA.
AC   A0A1Y4IFM5;
DT   30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=2,5-diketo-D-gluconic acid reductase {ECO:0000313|EMBL:OUP16461.1};
GN   ORFNames=B5F29_14680 {ECO:0000313|EMBL:OUP16461.1};
OS   Lachnoclostridium sp. An196.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX   NCBI_TaxID=1965583 {ECO:0000313|EMBL:OUP16461.1, ECO:0000313|Proteomes:UP000199780};
RN   [1] {ECO:0000313|Proteomes:UP000199780}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=An196 {ECO:0000313|Proteomes:UP000199780};
RA   Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA   Rychlik I.;
RT   "Function of individual gut microbiota members based on whole genome
RT   sequencing of pure cultures obtained from chicken caecum.";
RL   Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OUP16461.1}.
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DR   EMBL; NFJZ01000015; OUP16461.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Y4IFM5; -.
DR   OrthoDB; 9804790at2; -.
DR   Proteomes; UP000199780; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR   CDD; cd19071; AKR_AKR1-5-like; 1.
DR   Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR   PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000199780}.
FT   DOMAIN          17..258
FT                   /note="NADP-dependent oxidoreductase"
FT                   /evidence="ECO:0000259|Pfam:PF00248"
FT   ACT_SITE        49
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT   BINDING         107
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT   SITE            74
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ   SEQUENCE   287 AA;  32725 MW;  D6F5E34BCD98558F CRC64;
     MEQYFVLENG VKLPKIGYGT YKSTDGSGGR IIHMALEAGY RLLDTAAVYE NETEVGTAIR
     ESGVPREEIF LTSKVWKDRL GYEATKKSFE ESLERLQTDY LDLFLIHWPK PDPDSKDWKE
     LDRGSWAAME EFYHQGKVKA IGLSNFLPHH IEALLETAKV RPMVNQLELH VGYMQELAVQ
     YCKSQHIQVQ AWSPLGRRRV LEEPLVAKMA EKYGLTPAQF LLTFLLEQGI GVIPKASSME
     RLKQNLELPD VQIAEEDLYL LRCLPQIGWG GEHPDLPRVQ EQKGLGV
//