ID A0A1Y4MCI0_9FIRM Unreviewed; 210 AA.
AC A0A1Y4MCI0;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Redox-sensing transcriptional repressor Rex {ECO:0000256|HAMAP-Rule:MF_01131};
GN Name=rex {ECO:0000256|HAMAP-Rule:MF_01131};
GN ORFNames=B5F12_02000 {ECO:0000313|EMBL:OUP65699.1};
OS Pseudoflavonifractor sp. An176.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Pseudoflavonifractor.
OX NCBI_TaxID=1965572 {ECO:0000313|EMBL:OUP65699.1, ECO:0000313|Proteomes:UP000196146};
RN [1] {ECO:0000313|Proteomes:UP000196146}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=An176 {ECO:0000313|Proteomes:UP000196146};
RA Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA Rychlik I.;
RT "Function of individual gut microbiota members based on whole genome
RT sequencing of pure cultures obtained from chicken caecum.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Modulates transcription in response to changes in cellular
CC NADH/NAD(+) redox state. {ECO:0000256|HAMAP-Rule:MF_01131}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01131}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01131}.
CC -!- SIMILARITY: Belongs to the transcriptional regulatory Rex family.
CC {ECO:0000256|HAMAP-Rule:MF_01131}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUP65699.1}.
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DR EMBL; NFKO01000002; OUP65699.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y4MCI0; -.
DR Proteomes; UP000196146; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0051775; P:response to redox state; IEA:InterPro.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR HAMAP; MF_01131; Rex; 1.
DR InterPro; IPR003781; CoA-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR009718; Rex_DNA-bd_C_dom.
DR InterPro; IPR022876; Tscrpt_rep_Rex.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR35786; REDOX-SENSING TRANSCRIPTIONAL REPRESSOR REX; 1.
DR PANTHER; PTHR35786:SF1; REDOX-SENSING TRANSCRIPTIONAL REPRESSOR REX; 1.
DR Pfam; PF02629; CoA_binding; 1.
DR Pfam; PF06971; Put_DNA-bind_N; 1.
DR SMART; SM00881; CoA_binding; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01131};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_01131};
KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|HAMAP-Rule:MF_01131};
KW Reference proteome {ECO:0000313|Proteomes:UP000196146};
KW Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|HAMAP-Rule:MF_01131};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_01131};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_01131}.
FT DOMAIN 79..180
FT /note="CoA-binding"
FT /evidence="ECO:0000259|SMART:SM00881"
FT DNA_BIND 16..55
FT /note="H-T-H motif"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01131"
FT BINDING 90..95
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01131"
SQ SEQUENCE 210 AA; 23109 MW; E69FA6305F8AD7B1 CRC64;
MKKDKISSAV IRRLPRYYRH LDDLYRAGTI RISSGALAQS MGLTASQIRQ DLSCFGGFGQ
QGYGYNVERL RGEVAEILGM NRGHTVLILG AGNLGRALIE NFPFRANGFR LVAAFDVNPD
LVDTKLGGVP VYHVDGLDTF VREHRVSVAV LTVPGSVAVA TARRLSKLGV DGIWNFTNVE
LPEQEMPDVK VENVHFADSL LTLSYMISEE
//
