ID A0A1Y4N415_9FIRM Unreviewed; 1143 AA.
AC A0A1Y4N415;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=B5F09_09120 {ECO:0000313|EMBL:OUP75638.1};
OS Erysipelatoclostridium sp. An173.
OC Bacteria; Bacillota; Erysipelotrichia; Erysipelotrichales;
OC Coprobacillaceae; Thomasclavelia.
OX NCBI_TaxID=1965571 {ECO:0000313|EMBL:OUP75638.1, ECO:0000313|Proteomes:UP000196550};
RN [1] {ECO:0000313|Proteomes:UP000196550}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=An173 {ECO:0000313|Proteomes:UP000196550};
RA Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA Rychlik I.;
RT "Function of individual gut microbiota members based on whole genome
RT sequencing of pure cultures obtained from chicken caecum.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUP75638.1}.
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DR EMBL; NFKR01000019; OUP75638.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y4N415; -.
DR Proteomes; UP000196550; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000196550}.
FT DOMAIN 617..778
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 799..953
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1143 AA; 131396 MW; A57F9F24B81DAD88 CRC64;
MKKIDEILKK NLAFKTLLNG QGSIVVNNVN DEALLIAGAF LHLKKDIVVI KPNQYEANLL
YQQLALINEQ DCLFFPVDES YRIETLASSP ELLGQRIDAL YQLSQNKPKI LITHGQALVR
YLPTKQLFCD NCLDIKVGDH IDIYQLQTKL INAGYQNAMR VDQPFYFSKR GGVIDVFSIQ
YENPVRIEFF DDEIDNIRFY DQNSQRTIEH VNQITIIPAS DILYEEKEVR AVLGKINDLR
DQQFEELEEM YQDEYLNKVA IDQENLRNHD TSFNMYSYYN LFNQTANLLE YVNDPLIIQV
NRHDIEIAYK NYLEENFYYY QELASIGKSV KGLNLFSDLY QILDQDAVFF NPFAKDDKDV
FFNARGIMLD NNDENLLIKE IRDYLKVSKV LIALDDEHQI KLMCELFDRH EMLYTLVGIK
DELYPGLNIG IYRLNAGIEL VDEKLVIISA NELFKTRNLN KPKYLKYKNA KIIKDYQELN
VGDYVVHDNY GIGQYLGIKT LEVQGFHKDY LYVAYANNDT LYIPVEQFKL IRKYSSSEGK
IPKVNKLGSS NWQKTKAKAR NKVDDIADKL IEIYSARINQ PGFAFPPDDQ IQLEFEQAFG
YELTPDQQRS VDEIKADMEK PTPMDRLLCG DVGFGKTEVA LRAAFKAILA NKQVAFLCPT
TILSMQHYKT MEARFKDFPV RIALLNRFTS SKNKKQILSD LKQGNIDLLV GTHRILSKDV
IFKDIGLLCI DEEQRFGVRQ KERIKEYRKT IDVLTLTATP IPRTLQMSLM GIRGLSQIET
PPKNRQPVQT YVVGKSNVLI KQIIERELAR DGQVFYLYNR TNQIANVAYN ISLAVPQAKV
AIGHGQMDKN ELEDVMMRFV NKEFNVLVCT TIIETGIDIP NANTIIVEDA DRFGLSQLYQ
IKGRVGRSNR GAYAYLLYNP GKILNEEASK RLKAIKEFTE LGSGYKIAMR DLAIRGAGDI
LGGTQSGFID SIGFDMYMKI LQEAVNEKMG KVNEEKEIKS VNVNVDGYIP HDYVSSDIEK
LELYQRLDQA KTIVAIDHLK SEFIDYYGKL PKEVAVLIEK RKLDILANSE IIEDIVEVKG
KMQVTFTKQY SKELKGDQLF ELVNRLFTRP GFKQSGGQVM LLLPKSDQWL ERINQLISTL
SAS
//
