ID A0A1Y4NDC6_9FIRM Unreviewed; 300 AA.
AC A0A1Y4NDC6;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=B5F09_01340 {ECO:0000313|EMBL:OUP78827.1};
OS Erysipelatoclostridium sp. An173.
OC Bacteria; Bacillota; Erysipelotrichia; Erysipelotrichales;
OC Coprobacillaceae; Thomasclavelia.
OX NCBI_TaxID=1965571 {ECO:0000313|EMBL:OUP78827.1, ECO:0000313|Proteomes:UP000196550};
RN [1] {ECO:0000313|Proteomes:UP000196550}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=An173 {ECO:0000313|Proteomes:UP000196550};
RA Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA Rychlik I.;
RT "Function of individual gut microbiota members based on whole genome
RT sequencing of pure cultures obtained from chicken caecum.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUP78827.1}.
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DR EMBL; NFKR01000002; OUP78827.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y4NDC6; -.
DR Proteomes; UP000196550; Unassembled WGS sequence.
DR GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600:SF71; LARGE SUBUNIT PSEUDOURIDINE SYNTHASE, RLUD SUBFAMILY, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW Reference proteome {ECO:0000313|Proteomes:UP000196550}.
FT DOMAIN 92..242
FT /note="Pseudouridine synthase RsuA/RluA-like"
FT /evidence="ECO:0000259|Pfam:PF00849"
FT ACT_SITE 138
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 300 AA; 34914 MW; 24AF290FDB4FC6B0 CRC64;
MKYQIKGDYL IIEINDDFVG MSVEQFFNYF HLSRKTIHLL KQNKQYALNN NFVTSNTLLK
QGDSLKVLAF DQGIDYQFQS YPLTVVYEDD FILIVNKPIN TAIYPEDKNG LNTLCNYVAN
YYLETNQYIP VRHIHRLDKD TTGLVMFCKC AFLQPKLDIM MANKEIKRTY LAIVDGIVKK
DLIINKPISR DRHQNRMRTS NNGKKAVTKV HVLKRSFPSN YTVVKCDLQT GRKHQIRVHL
SSINHPLLSD PLYGTNSKLI NRVALHGYKL EFNHPFFNQK ITVFADIPDD MKFIGKIDLQ
//
