ID A0A1Y4TEX1_9FIRM Unreviewed; 520 AA.
AC A0A1Y4TEX1;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=B5E66_01585 {ECO:0000313|EMBL:OUQ40595.1};
OS Faecalibacterium sp. An121.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Faecalibacterium.
OX NCBI_TaxID=1965550 {ECO:0000313|EMBL:OUQ40595.1, ECO:0000313|Proteomes:UP000196164};
RN [1] {ECO:0000313|Proteomes:UP000196164}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=An121 {ECO:0000313|Proteomes:UP000196164};
RA Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA Rychlik I.;
RT "Function of individual gut microbiota members based on whole genome
RT sequencing of pure cultures obtained from chicken caecum.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUQ40595.1}.
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DR EMBL; NFLM01000002; OUQ40595.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y4TEX1; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000196164; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000196164};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 13..164
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 178..486
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 520 AA; 58382 MW; 8186A92CE9F7B64D CRC64;
MVDMKKEQER DELHRAIWAI ADALRGSVDG WDFKAYVLGM MFYRYISENL TNYINEGEWE
AGNDGFDYTQ LSDEDAEQAR KDMINTKGFF MVPSELFCNV RARAGKDENL NMTLEAAFRH
IEESAKGSPS EDNFAGLFDD LDVNSNKLGA TVAKRNANLV KLMNGIADMK LGDYKDNSID
AFGDAYEYLM GMYASNAGKS GGEFFTPQEV SELLTRMAVA GKTEVNKVYD PACGSGSLLL
KAAKILGKDN VRQGFFGQEI NLTTYNLCRI NMFLHDIDYD KFDIAHEDTL VNPQHWDDEP
FEVIVSNPPY SIKWEGDGNP VLINDPRFSP AGVLAPKSKA DLAFIMHSLA WLATNGTAAI
VCFPGIMYRG GAEKKIRQYL IDNNFIDCII QLPSNLFYGT SIATCIIVLK KSKADNRTLF
IDASAECIKV TNNNKLTEEN IRRIVDEFVS RADVDHFARC VPYEEIAQNE YNLSVSTYVQ
PEDTREVIDI VKLNAEIEQI VAREQVLRDE IAKIIGEIEG
//
