ID A0A1Y4TWF7_9FIRM Unreviewed; 579 AA.
AC A0A1Y4TWF7;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=B12-binding domain-containing radical SAM protein {ECO:0000313|EMBL:OUQ49872.1};
GN ORFNames=B5E62_09350 {ECO:0000313|EMBL:OUQ49872.1};
OS Lachnoclostridium sp. An118.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=1965547 {ECO:0000313|EMBL:OUQ49872.1, ECO:0000313|Proteomes:UP000199786};
RN [1] {ECO:0000313|Proteomes:UP000199786}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=An118 {ECO:0000313|Proteomes:UP000199786};
RA Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA Rychlik I.;
RT "Function of individual gut microbiota members based on whole genome
RT sequencing of pure cultures obtained from chicken caecum.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUQ49872.1}.
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DR EMBL; NFLQ01000009; OUQ49872.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y4TWF7; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000199786; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR025288; DUF4080.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13311; DUF4080; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000199786}.
FT DOMAIN 2..145
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 183..406
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 579 AA; 67652 MW; 9B6148F118531389 CRC64;
MRKIVLVAVN AKYIHSNLAV YSLKAYADAR LRRRKSPASG TVEIEIGEYT INQQPDGIME
DIYKRRPDLL CFSCYIWNIR MIRELLPELG KVLPDTEIWL GGPEVSYDPV RLLEQLPQVT
GIMKGEGEET FAVLAERKTD PGADLADVEG ITWRDSLGQI QDHPWRQPLD LDEIPFVYQD
MTDFRHRIVY YESSRGCPFS CSYCLSSIDK RIRFRSLQLV KKELQFFLDA GTGQVKFVDR
TFNCRHDHAL EIWKYISSHD NGVTNFHFEI SADLLNGEEL ALIRSMRPGL IQLEIGVQST
NPETIKEIRR TMDLGRLEEN VKRLREGRNV HQHLDLIAGL PQEDLESFSR SFDRVYALRP
DQLQLGFLKV LKGSRMETMK EAYGLLCRDI PPYEVLSTRW LSYGDTLILK GVEEMTELYY
NSGQFRTALE LLERREPSPF ALYRRLADFY EKRALDQMQH ARAVRYEILL DFAGENWPED
AEEMRERLIF DYYLRENAKS RPAFAGESRA DSRWVRSFYE TEAREHRYLT GCGGMDKNQL
RRMTHLEFFP LKGEYVLFDY RERDPLTNDG RGIHIAIEK
//
