ID A0A1Y6L745_9GAMM Unreviewed; 728 AA.
AC A0A1Y6L745;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=enoyl-CoA hydratase {ECO:0000256|ARBA:ARBA00012076};
DE EC=4.2.1.17 {ECO:0000256|ARBA:ARBA00012076};
GN Name=fadB {ECO:0000313|EMBL:SMY18328.1};
GN ORFNames=PAQU9191_03669 {ECO:0000313|EMBL:SMY18328.1};
OS Photobacterium aquimaris.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Photobacterium.
OX NCBI_TaxID=512643 {ECO:0000313|EMBL:SMY18328.1, ECO:0000313|Proteomes:UP000196485};
RN [1] {ECO:0000313|Proteomes:UP000196485}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=type strain: CECT 9192 {ECO:0000313|Proteomes:UP000196485};
RA Rodrigo-Torres L., Arahal R. D., Lucena T.;
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000256|RuleBase:RU003707}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC hydratase/isomerase family. {ECO:0000256|ARBA:ARBA00008750}.
CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
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DR EMBL; FYAH01000014; SMY18328.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y6L745; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000196485; Unassembled WGS sequence.
DR GO; GO:0036125; C:fatty acid beta-oxidation multienzyme complex; IEA:InterPro.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:InterPro.
DR GO; GO:0004165; F:delta(3)-delta(2)-enoyl-CoA isomerase activity; IEA:InterPro.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:InterPro.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR012799; FadB.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR02437; FadB; 1.
DR PANTHER; PTHR43612:SF8; FATTY ACID OXIDATION COMPLEX SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR Pfam; PF00725; 3HCDH; 2.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00067; 3HCDH; 1.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000196485}.
FT DOMAIN 320..497
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 499..595
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
FT DOMAIN 630..693
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 728 AA; 78666 MW; 6A25556D3B4DB107 CRC64;
MIYHGETVSV NYLEDGIAEV IFDAPSPVNK FDLATLESFN LALDALYQQP KLKGVILRST
KTAFIVGADI TEFLGLFALS ADQLSKWLHH ANTIFNKLED LPVPTIAAIN GYALGGGCEC
VLATDFRVAD HQARIGLPET KLGIMPGFGG TVRLPRLIGA DSAMEIITAG KDKTAAAALK
IGLIDAVVAT EMLPTAALAM IKQAIAGDLD WQQRRHQKQA PLPLSTTEAL MSFTTAKAMV
ANIAGKHYLA PIAAVTAIET AANCNRDPAL AIENKHFVTL AKTDIAQALV GIFLNDQYIK
NQAKQAKQAI KIAPATQQAM VLGAGIMGGG IAYQSATHHV PVIMKDIAAD ALTLGMEQAA
KLLNKKLTRG RIDGLQMAAT LATITPTLTY SDSCHVDIVI EAVIENPTIK ATVLAEIEQQ
VDATTVLTSN TSTIPINLLA QSLQRPQNFC GMHFFNPVHK MPLVEIIRGQ HTSNETIDRV
VAYAAKMGKT PIVVNDCPGF FVNRVLFPYL AAFSLLLRDG GDYVQIDTIM EQDFGWPMGP
AYLLDVVGID TAHHAQKVMA QGFPQRMAKN YTDAVDVLYQ HQRFGQKTGA GFFNYSTDKK
GKPKKIFDPQ TATLLQPIAA TPTDFDSTVV IDRMLIPMIN EVVRCLEENI IATPAEADMA
LVYGLGFPPF RGGVFRYLDH IGLANYVALA DSYQSLGALY QVPAGLRAKA AADECYYPTA
TAVTMTHH
//
