ID A0A1Y9H1Y9_9DIPT Unreviewed; 733 AA.
AC A0A1Y9H1Y9;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=Acetylcholinesterase {ECO:0000256|ARBA:ARBA00020419};
DE EC=3.1.1.7 {ECO:0000256|ARBA:ARBA00013276};
OS Anopheles dirus.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=7168 {ECO:0000313|EnsemblMetazoa:ADIR015679-PA, ECO:0000313|Proteomes:UP000075884};
RN [1] {ECO:0000313|Proteomes:UP000075884}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WRAIR2 {ECO:0000313|Proteomes:UP000075884};
RG The Broad Institute Genomics Platform;
RA Neafsey D.E., Walton C., Walker B., Young S.K., Zeng Q., Gargeya S.,
RA Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Anopheles dirus WRAIR2.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:ADIR015679-PA}
RP IDENTIFICATION.
RC STRAIN=WRAIR2 {ECO:0000313|EnsemblMetazoa:ADIR015679-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- FUNCTION: Rapidly hydrolyzes choline released into the synapse.
CC {ECO:0000256|ARBA:ARBA00037263}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetylcholine + H2O = acetate + choline + H(+);
CC Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7;
CC Evidence={ECO:0000256|ARBA:ARBA00000347};
CC -!- SUBCELLULAR LOCATION: Synapse {ECO:0000256|ARBA:ARBA00034103}.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000256|ARBA:ARBA00005964}.
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DR AlphaFoldDB; A0A1Y9H1Y9; -.
DR STRING; 7168.A0A1Y9H1Y9; -.
DR ESTHER; anoga-ACHE1; ACHE.
DR EnsemblMetazoa; ADIR015679-RA; ADIR015679-PA; ADIR015679.
DR VEuPathDB; VectorBase:ADIR015679; -.
DR OrthoDB; 4386at2759; -.
DR Proteomes; UP000075884; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
DR GO; GO:0003990; F:acetylcholinesterase activity; IEA:UniProtKB-EC.
DR CDD; cd00312; Esterase_lipase; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR InterPro; IPR000997; Cholinesterase.
DR PANTHER; PTHR43918; ACETYLCHOLINESTERASE; 1.
DR PANTHER; PTHR43918:SF12; ACETYLCHOLINESTERASE 1; 1.
DR Pfam; PF00135; COesterase; 1.
DR PRINTS; PR00878; CHOLNESTRASE.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Neurotransmitter degradation {ECO:0000256|ARBA:ARBA00022867};
KW Serine esterase {ECO:0000256|ARBA:ARBA00022487};
KW Signal {ECO:0000256|SAM:SignalP}; Synapse {ECO:0000256|ARBA:ARBA00023018};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..40
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 41..733
FT /note="Acetylcholinesterase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011005351"
FT TRANSMEM 711..732
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 162..682
FT /note="Carboxylesterase type B"
FT /evidence="ECO:0000259|Pfam:PF00135"
FT REGION 120..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..156
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 356
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
FT ACT_SITE 482
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
FT ACT_SITE 596
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
SQ SEQUENCE 733 AA; 80057 MW; C7CE10639205F0E3 CRC64;
MEIRGLLMAR LRLGRRQVPL QVLCASALLL LLPPSALVHG RHHELNNGAA IGAHQLSAAG
GAASLTSQPS GSAGSLAAGA AAAAVAAGAG SSASSSLSSS SAEDDVARIT LSKDAGHGES
VRIIDPELGT LEREHVHSGA TPRRRGLTRR ESNADANDND PLVVNTDKGR IRGITVEAPS
GKKVDVWLGI PYAQPPVGPL RFRHPRPAEK WTGVLNATTP PNSCVQIVDT VFGDFPGATM
WNPNTPLSED CLYINVVAPR PRPKNAAVML WIFGGGFYSG TATLDVYDHR ALASEENVIV
VSLQYRVASL GFLFLGTPEA PGNAGLFDQN LALRWVRDNI HRFGGDPSRV TLFGESAGAV
SVSLHLLSAL SRDLFQRAIL QSGSPTAPWA LVSREEATLR ALRLAEAVGC PHEPSKLSES
VECLRGKDPH VLVNNEWGTL GICEFPFVPV VDGAFLDETP QRSLASGRFK KTEILTGSNT
EEGYYFIIYY LTELLRKEEG VTVSREEFLQ AVRELNPYVN GAARQAIVFE YTDWTEPDNP
NSNRDALDKM VGDYHFTCNV NEFAQRYAEE GNNVYMYLYT HRSKGNPWPR WTGVMHGDEI
NYVFGEPLNP TLGYTEDEKD FSRKVMRYWS NFAKTGNPNP STASSEFPEW PKHTAHGRHY
LELGLNTSFV GRGPRLRQCA FWKKYLPQLV AATSNLQVAA PPSAPCESSA FFYRPNLIVL
LVALLSVAIR FIQ
//
