ID A0A1Z5IHK4_9LACO Unreviewed; 886 AA.
AC A0A1Z5IHK4;
DT 27-SEP-2017, integrated into UniProtKB/TrEMBL.
DT 27-SEP-2017, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460,
GN ECO:0000313|EMBL:GAX01233.1};
GN ORFNames=IWT126_01259 {ECO:0000313|EMBL:GAX01233.1};
OS Secundilactobacillus silagei JCM 19001.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Secundilactobacillus.
OX NCBI_TaxID=1302250 {ECO:0000313|EMBL:GAX01233.1, ECO:0000313|Proteomes:UP000198402};
RN [1] {ECO:0000313|EMBL:GAX01233.1, ECO:0000313|Proteomes:UP000198402}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IWT126 {ECO:0000313|EMBL:GAX01233.1,
RC ECO:0000313|Proteomes:UP000198402};
RA Tohno M., Tanizawa Y., Arita M.;
RT "Draft genome sequences of new species of the genus Lactobacillus isolated
RT from orchardgrass silage.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541, ECO:0000256|RuleBase:RU004460}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAX01233.1}.
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DR EMBL; BCMG01000005; GAX01233.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Z5IHK4; -.
DR STRING; 1302250.GCA_001313225_01705; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000198402; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 5..268
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 303..477
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 644..850
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 886 AA; 100247 MW; AC8BC63E56A400BB CRC64;
MTESKRLLLI DGNSVAFRAF FALYTSLNSF TNADGLHTNA IFGFNRMLDI MLEQVKPTEA
LVAFDAGKVT FRTKKYADYK GGRSKTPPEL LEQMPVIRQL LTARGIKSYE LKNYEADDII
GTMAKQAEEA GYETTIFTGD RDLTQLTSDK TSVAVSKKGV TEVETYTPEH VEEKLGVRPN
QIIEIKGLQG DTSDNYPGVT GVGPKTAVSL IQKYDTIDGV YDNLDKITAK KMKEHLIEDK
NSAFQSRDLA TIRRDAPIEI KLSDLAYKGD QRDELVEFYQ EMNFQSFLNK MNADSGDEDV
LGLKPVKFTV LTADHLSDLD QFNDHVSFYL EMPEANYHTS TFAGFVIGEG DKWFVSRDVD
LLKQAPLKAL LSGEQVQKDV FDVKRTYVGL NRLGIHLAHV DFDLLLASYL LDTNENSNDL
GRLAFQHGYH QIQSDEEVYG KGAKRAIPDD DQIFFEHLTR KGITIEALKK SLFKQLDNNE
QTQLYRDLEL PMAYVLAHME IAGIMVDTDR LTAMQSEFTE LLAQTQQTIF QEAGEEFNIG
SPKQLQHILF EKLGLTPIKK TKTGYSTSVD VLEKLAAEAP IVENILKYRQ ISKIQSTYVE
GLLKVVHSTD QKVHTRYLQT LTQTGRLSSV DPNLQNIPIR TEEGRRIRQA FIPSHKGWQI
FSSDYSQIEL RVLAHITHDA NLQEAFREGE DIHASTARRI FHLGPDAEID PNMRRQAKAV
NFGIVYGISD FGLSQNIGIT RKQARQFIDT YFEEYPGVKK YMTDIVASAK DKGYVETIAH
RRRYLPDINA RNFNQRSFAE RTAMNTPIQG SAADIIKIAM INMEDAIKDL QATMLLQVHD
ELIFEAPAEE IPTLEKLVPS VMDSAVKLEI PLKVESHYGN TWYEAK
//
