ID A0A1Z5JYK9_FISSO Unreviewed; 581 AA.
AC A0A1Z5JYK9;
DT 27-SEP-2017, integrated into UniProtKB/TrEMBL.
DT 27-SEP-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=5'-nucleotidase {ECO:0008006|Google:ProtNLM};
GN ORFNames=FisN_3Lh063 {ECO:0000313|EMBL:GAX19123.1};
OS Fistulifera solaris (Oleaginous diatom).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Bacillariophyceae; Bacillariophycidae; Naviculales; Naviculaceae;
OC Fistulifera.
OX NCBI_TaxID=1519565 {ECO:0000313|EMBL:GAX19123.1, ECO:0000313|Proteomes:UP000198406};
RN [1] {ECO:0000313|EMBL:GAX19123.1, ECO:0000313|Proteomes:UP000198406}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JPCC DA0580 {ECO:0000313|EMBL:GAX19123.1,
RC ECO:0000313|Proteomes:UP000198406};
RX PubMed=25634988; DOI=10.1105/tpc.114.135194;
RA Tanaka T., Maeda Y., Veluchamy A., Tanaka M., Abida H., Marechal E.,
RA Bowler C., Muto M., Sunaga Y., Tanaka M., Yoshino T., Taniguchi T.,
RA Fukuda Y., Nemoto M., Matsumoto M., Wong P.S., Aburatani S., Fujibuchi W.;
RT "Oil accumulation by the oleaginous diatom Fistulifera solaris as revealed
RT by the genome and transcriptome.";
RL Plant Cell 27:162-176(2015).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR017434-2};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR017434-2};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000256|ARBA:ARBA00009589}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAX19123.1}.
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DR EMBL; BDSP01000134; GAX19123.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Z5JYK9; -.
DR InParanoid; A0A1Z5JYK9; -.
DR OrthoDB; 3626840at2759; -.
DR Proteomes; UP000198406; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR NCBIfam; TIGR02244; HAD-IG-Ncltidse; 1.
DR PANTHER; PTHR12103; 5'-NUCLEOTIDASE DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR12103:SF15; CYTOSOLIC PURINE 5'-NUCLEOTIDASE; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR017434-2};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR017434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000198406};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..581
FT /note="5'-nucleotidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012306392"
FT ACT_SITE 101
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT ACT_SITE 103
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT BINDING 101
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 103
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 409
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
SQ SEQUENCE 581 AA; 67734 MW; 01EBCDABC0E649E3 CRC64;
MIFFSLLLLS LSFQDVFTFT AFSRKGVLPT LRSQVDDHTT TTKTTTKINE NEGGRYDKLL
KELNLDLPAL ARLPKKHTIT PFDVFCNREL KLEGIQAIGF DMDYTLAQYN ETTFAELAFD
GAKRKLVESL GYPEEVLDFT YDPAHWVRGL IIDTQRGNFL KIDRHKYVRV ARHGFEVISS
TTRKHLYSRQ FNKVMSFTEK QFVNMDTLFQ FVDAHLFASL IELKDKGDYE ELDYKTYEEI
YRQVRECVDL CHRDGVIKDQ VHLNPEPYVI FDKGMVPMLR RFRQEGIKLF LLTNSLWEYT
STVMNYLYHG ERVPDERQKD NEWMELFDLV ITGACKPAYM VDSHLNLFRV DPSTGTLRNT
DGVYEIEALG PNGATKFLQK GKTFQGGNWL HLQAMLEIEA GEEILYVGDH LYSDVLRSKR
TLGWRSVFIM PELEDEMKTF AANAPLARQI IELRKLREEL GVLGEVLRRQ ADNPQKEQEL
NHLNDQDDLL KEKLIEMTSR WHAAFHPIWG AMFHAGYQDS RFAFYVQNYA CVYTSRASNL
GLWSKQRSFR TSMEMLSHDR LLFATDHTDF AEVDPWTHIA E
//
