ID A0A1Z5JZF0_FISSO Unreviewed; 777 AA.
AC A0A1Z5JZF0;
DT 27-SEP-2017, integrated into UniProtKB/TrEMBL.
DT 27-SEP-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=sn-1-specific diacylglycerol lipase {ECO:0000256|ARBA:ARBA00026104};
DE EC=3.1.1.116 {ECO:0000256|ARBA:ARBA00026104};
GN ORFNames=FisN_4Lh373 {ECO:0000313|EMBL:GAX19394.1};
OS Fistulifera solaris (Oleaginous diatom).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Bacillariophyceae; Bacillariophycidae; Naviculales; Naviculaceae;
OC Fistulifera.
OX NCBI_TaxID=1519565 {ECO:0000313|EMBL:GAX19394.1, ECO:0000313|Proteomes:UP000198406};
RN [1] {ECO:0000313|EMBL:GAX19394.1, ECO:0000313|Proteomes:UP000198406}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JPCC DA0580 {ECO:0000313|EMBL:GAX19394.1,
RC ECO:0000313|Proteomes:UP000198406};
RX PubMed=25634988; DOI=10.1105/tpc.114.135194;
RA Tanaka T., Maeda Y., Veluchamy A., Tanaka M., Abida H., Marechal E.,
RA Bowler C., Muto M., Sunaga Y., Tanaka M., Yoshino T., Taniguchi T.,
RA Fukuda Y., Nemoto M., Matsumoto M., Wong P.S., Aburatani S., Fujibuchi W.;
RT "Oil accumulation by the oleaginous diatom Fistulifera solaris as revealed
RT by the genome and transcriptome.";
RL Plant Cell 27:162-176(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:33275, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:28868; EC=3.1.1.116;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33276;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAX19394.1}.
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DR EMBL; BDSP01000136; GAX19394.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Z5JZF0; -.
DR InParanoid; A0A1Z5JZF0; -.
DR OrthoDB; 373802at2759; -.
DR Proteomes; UP000198406; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd00519; Lipase_3; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR PANTHER; PTHR45792; DIACYLGLYCEROL LIPASE HOMOLOG-RELATED; 1.
DR PANTHER; PTHR45792:SF8; DIACYLGLYCEROL LIPASE-ALPHA; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000198406};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 31..53
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 84..105
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 126..151
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 460..602
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
FT REGION 268..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 777 AA; 87480 MW; 3C1699C521198F42 CRC64;
MRLDVVRAGG FIEYLLYDPN DQDSTCRNSH FYCLLTLIYA LLTALYIIAA IILDQRIAFW
SSQGSPTERE PRSSKVAHLL ELKLLPVTIV LLLVWATGLS TISTGKRYYR CVYESELAHD
PTSTPVVYWW VPLVILLSLQ FTEMLFSWRF LFGLCRQPRV INSASWHGDE EAYLTDPVNH
ELIEEMWAER CASFCQCLSW MSCFLFGGRE LAGQAEFGDV ARALADYLEY RGVLDVVPSD
IVTGLILVRN IQQERILNYR RELRSKATST LSTASTPEGR TYTTETGEDS GLLGSDTVSP
INLPAAISST QPLMDEIDPL DPADRNGRLL DRNSFSDMQC LEEGARYASY ALAIYTWVLY
VYERPISGPF ELLGRGCIAC CRGKYKRAHS PDGLNISGEN EIVEIGEAEF HGDNMCRTHR
NALLLSANLS DADLVYAQLR SGFRDNPYCI ILDHAWKTVV VSIRGTFSLE DCVTDVLIQP
ESLEKLGVDF GFNGHGQFCH GGVMNCVRNI YRDLERHHLL ERLLLGPNAA YPDYSLRIVG
HSLGAATGTL LSYMLRNKYP NLRCLNYSPV GCAFTWDLAT GCSEWCDSFF VDSDIVPRLS
LESMDKLRDD MLEGIGRLKV PKIHVARRAV GHYGLLACGD FCYRFFSDEH ALQDTAELLH
EPENVPDSAF QDQLSRFKLM QAERKRSRGV IRSTKLYPPG RMIHLAKIGE KRSCLHGCAR
CLTCCSTSFG SEYVPVWVRN DELHEIVVSP TMGTDHFPNR LRKILVDVAS SHGMNAS
//
