ID A0A1Z8L5M0_9PROT Unreviewed; 444 AA.
AC A0A1Z8L5M0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE SubName: Full=tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB {ECO:0000313|EMBL:OUT44307.1};
GN ORFNames=CBB73_04275 {ECO:0000313|EMBL:OUT44307.1};
OS Pelagibacteraceae bacterium TMED13.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus Pelagibacterales;
OC Candidatus Pelagibacteraceae.
OX NCBI_TaxID=1986627 {ECO:0000313|EMBL:OUT44307.1, ECO:0000313|Proteomes:UP000196466};
RN [1] {ECO:0000313|EMBL:OUT44307.1, ECO:0000313|Proteomes:UP000196466}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TMED13 {ECO:0000313|EMBL:OUT44307.1};
RA Tully B.J., Sachdeva R., Graham E.D., Heidelberg J.F.;
RT "290 Metagenome-assembled Genomes from the Mediterranean Sea: a resource
RT for marine microbiology.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUT44307.1}.
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DR EMBL; NHBS01000027; OUT44307.1; -; Genomic_DNA.
DR Proteomes; UP000196466; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035596; F:methylthiotransferase activity; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR Gene3D; 3.40.50.12160; Methylthiotransferase, N-terminal domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR038135; Methylthiotransferase_N_sf.
DR InterPro; IPR006463; MiaB_methiolase.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; TIGR01574; miaB-methiolase; 1.
DR NCBIfam; TIGR00089; MiaB/RimO family radical SAM methylthiotransferase; 1.
DR PANTHER; PTHR43020; CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1; 1.
DR PANTHER; PTHR43020:SF2; MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SFLD; SFLDF00273; (dimethylallyl)adenosine_tRNA; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OUT44307.1}.
FT DOMAIN 3..123
FT /note="MTTase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51449"
FT DOMAIN 143..379
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 444 AA; 50721 MW; 0535188FD98D724B CRC64;
MKQSFFIKTY GCQMNVYDSE KIVELMTSGG YRQSSNPEES DIAIFNTCHI REKAAEKLYS
DLGRISKYKE NKIKSGSSMK IIVTGCVAQA EGDEIKKRNK SVDLIFGPQN YQNITDALEG
KNEKYVYSDF LCADKFESLP IRNSHEIXXX VTIQEGCDKF CSFCVVPYTR GAEFSRPVEG
IFLEVKKLVE MGAKEVILLG QNVXSYNSNV FEDGIRVSVK LSKLCNLISN ITGLERIRYL
TSHPNDIEND LINEHYTNKK LMPFLHLPIQ SGSDKILKRM NRNHSRNDYM NLVRNIKSKI
NNMAFSSDFI VGYPGETDHD FNQTIDLIEH VGFASSYSFK YSPRPGTQSS LKHPNVVNDN
VSDLRLKKIQ EILNKQQSEF NLKFIDKNEE VLFTDKGKKD NQFVGRTKYL QPVHVLCNRN
IIGKKLEVXL ESLTSFSFHG KIVN
//
