ID A0A1Z9JEJ0_9CYAN Unreviewed; 465 AA.
AC A0A1Z9JEJ0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=23S rRNA (Uracil(1939)-C(5))-methyltransferase RlmD {ECO:0000313|EMBL:OUW29137.1};
GN ORFNames=CBD37_04080 {ECO:0000313|EMBL:OUW29137.1};
OS Cyanobacteria bacterium TMED177.
OC Bacteria; Cyanobacteriota.
OX NCBI_TaxID=1986677 {ECO:0000313|EMBL:OUW29137.1, ECO:0000313|Proteomes:UP000195337};
RN [1] {ECO:0000313|EMBL:OUW29137.1, ECO:0000313|Proteomes:UP000195337}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TMED177 {ECO:0000313|EMBL:OUW29137.1};
RA Tully B.J., Sachdeva R., Graham E.D., Heidelberg J.F.;
RT "290 Metagenome-assembled Genomes from the Mediterranean Sea: a resource
RT for marine microbiology.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUW29137.1}.
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DR EMBL; NHIA01000030; OUW29137.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Z9JEJ0; -.
DR Proteomes; UP000195337; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 13..71
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT ACT_SITE 418
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 418
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 294
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 324
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 345
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 391
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 465 AA; 50825 MW; 5FFE0841E516BD52 CRC64;
MPENNGAQAD PTAPKPGLSL ELDVVDLDRD GHGLARWNNW VIVVPGLLPG ERGQVQLQQR
QKSRWLSRVT ERLVSSPLRR HPPCILASDC GGCTLQHLED SAQRSWKQDN LAQALNRIGG
IDHPADPALA DQRGIGYRNR GLIPLRREDT GRLRMGYFRR GTHRIVNLSQ CPVLDPRLNG
LVAPLKADLD ASGLEADHDL SQGQGLRHLG LRLGHHTGEV LITVVSSQPH PALPLLAQRW
FHQWDHVKGV TLNLQPRRTN QILGPNTQVL AGVDRIREQF CGLDLFLSTT TFFQINTPQA
EQIVRLIVAW LGAKSVDGPI IDAYCGIGTI SLPLAAEGLQ VVGIEINPDS IAQAQANAVA
NGLQTLTRFR AGDVATELQA ELSGCSAVVL DPPRRGLAPD VVEAILTDPP PLLAYLSCDV
ATQARDLRQL LAPAGVYALE RLQPVDFFPQ TTHLESLALM RRINS
//
