ID A0A200PM55_9MAGN Unreviewed; 631 AA.
AC A0A200PM55;
DT 27-SEP-2017, integrated into UniProtKB/TrEMBL.
DT 27-SEP-2017, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=BVC80_717g9 {ECO:0000313|EMBL:OUZ99290.1};
OS Macleaya cordata.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Papaveraceae; Papaveroideae;
OC Macleaya.
OX NCBI_TaxID=56857 {ECO:0000313|EMBL:OUZ99290.1, ECO:0000313|Proteomes:UP000195402};
RN [1] {ECO:0000313|EMBL:OUZ99290.1, ECO:0000313|Proteomes:UP000195402}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. BLH2017 {ECO:0000313|Proteomes:UP000195402};
RC TISSUE=Root {ECO:0000313|EMBL:OUZ99290.1};
RX PubMed=28552780; DOI=.1016/j.molp.2017.05.007;
RA Liu X., Liu Y., Huang P., Ma Y., Qing Z., Tang Q., Cao H., Cheng P.,
RA Zheng Y., Yuan Z., Zhou Y., Liu J., Tang Z., Zhuo Y., Zhang Y., Yu L.,
RA Huang J., Yang P., Peng Q., Zhang J., Jiang W., Zhang Z., Lin K., Ro D.K.,
RA Chen X., Xiong X., Shang Y., Huang S., Zeng J.;
RT "The Genome of Medicinal Plant Macleaya cordata Provides New Insights into
RT Benzylisoquinoline Alkaloids Metabolism.";
RL Mol. Plant 10:975-989(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUZ99290.1}.
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DR EMBL; MVGT01004512; OUZ99290.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A200PM55; -.
DR InParanoid; A0A200PM55; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000195402; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF64; ENDOGLUCANASE 7; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059}; Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000195402};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 88..107
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 123..595
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 525
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 573
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 582
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 631 AA; 71250 MW; 7564CDF85F78AE5E CRC64;
MHSANHWGRG GSLEINKDSV AEDDERRRTE RNMMDLKNRG ALSHSHHQLQ LDETQQSWLL
GPQEKKKNKK RYVDFGSIVY HRTAFKCTLG YILIAFLVIG LPTTSIAKTL PKHKHHPPPP
DNYTVALKKA LLFFDAQMSG RLPKNNRVPW RGNSGLKDGS DLSDVKGGLV GGYYDAGDNI
KFHFPMAFSM SMLSWSVIEY SHKYKAIGEY EHVRDIIKWG TDYLLLTFNS SATTINKIYS
QVGVAWNDFT TPDDHYCWQR PEDMDYPRPV TTTFSGPDLA GEMAAALASA SIVFRDNTKY
SKKLIKGATT LFRFARDFRK RTSYSRGDVN IAMFYNSTGY YDEYMWGAAW MYYATGNSSY
LELATNKGIT KNAKAFSRIP DLSVLSWDNK LPAAQLLLTR MRIFLSPEYP YESTLQSYHN
MTGLNMCSYL RRFNVFNWTQ GGMIQLNHGR PQPLQFVVNA AFISSLYVDY MNATGVPGWF
CGPSYISSDV LLRFAISQVD YILGANPRNM SYLVGYGTRF PKHVHHRGAS IPRDGTRYSC
TGGWKWRDSH NPNPNTITGA MVGGPDKFDR FRDVRTNYNY TEPTLAGNAG LVAALVSLTS
SGGNHIDKNS IFSAVPPFYA TSPPPPPPWK P
//
