ID   A0A202E9V1_9EURY        Unreviewed;       353 AA.
AC   A0A202E9V1;
DT   27-SEP-2017, integrated into UniProtKB/TrEMBL.
DT   27-SEP-2017, sequence version 1.
DT   24-JAN-2024, entry version 16.
DE   SubName: Full=Endoglucanase {ECO:0000313|EMBL:OVE85031.1};
GN   ORFNames=B2G88_11810 {ECO:0000313|EMBL:OVE85031.1};
OS   Natronolimnobius baerhuensis.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales;
OC   Natrialbaceae; Natronolimnobius.
OX   NCBI_TaxID=253108 {ECO:0000313|EMBL:OVE85031.1, ECO:0000313|Proteomes:UP000196084};
RN   [1] {ECO:0000313|EMBL:OVE85031.1, ECO:0000313|Proteomes:UP000196084}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 1.3597 {ECO:0000313|EMBL:OVE85031.1,
RC   ECO:0000313|Proteomes:UP000196084};
RA   Zhao B.;
RT   "Natronthermophilus aegyptiacus gen. nov.,sp. nov., an aerobic, extremely
RT   halophilic alkalithermophilic archaeon isolated from the athalassohaline
RT   Wadi An Natrun, Egypt.";
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OVE85031.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; MWPH01000002; OVE85031.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A202E9V1; -.
DR   OrthoDB; 30642at2157; -.
DR   Proteomes; UP000196084; Unassembled WGS sequence.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF0; AMINOPEPTIDASE YPDE-RELATED; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000196084}.
FT   ACT_SITE        208
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         66
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         176
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         176
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         209
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         231
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         318
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   353 AA;  37470 MW;  B951C03B35DBE3EC CRC64;
     MNASSRDFLV ELLETPSPSG YETRGQRVWL DYVDQFADDV RTDAYGNAIA VHEGDPDAPE
     VVLTGHADEI GFIVRSIDDD GFVRPGRIGG SDPSVSQGQH VTIHAADGPV EGVVGQTAIH
     LRDESDDDPE ISDLWIDIGA ESETEASERL EIGDPITFSS TVSWLSETRL AARGLDNRVG
     TWAAAEGFRN AVENDTEATV YAVSTVQEEV GREGAKMVGF DLEPDAVVVV DVGHAVDYPS
     APSEKTSQME LGGGPALGRG SSNHPVLFEA LRSVAADREI DVQLEALGLG TGTDADGFFT
     AAGGIPSQVV SVPNRYMHTP VEVIDTDDLE DVATLLGGFA NRASEFAPFA VDI
//