ID A0A220TYZ4_9BACI Unreviewed; 243 AA.
AC A0A220TYZ4;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=RNA polymerase sigma factor SigI {ECO:0000256|HAMAP-Rule:MF_02064};
GN Name=sigI {ECO:0000256|HAMAP-Rule:MF_02064,
GN ECO:0000313|EMBL:ASK60911.1};
GN ORFNames=CFK37_01135 {ECO:0000313|EMBL:ASK60911.1};
OS Virgibacillus phasianinus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Virgibacillus.
OX NCBI_TaxID=2017483 {ECO:0000313|EMBL:ASK60911.1, ECO:0000313|Proteomes:UP000198312};
RN [1] {ECO:0000313|EMBL:ASK60911.1, ECO:0000313|Proteomes:UP000198312}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LM2416 {ECO:0000313|EMBL:ASK60911.1,
RC ECO:0000313|Proteomes:UP000198312};
RA Tak E.J., Bae J.-W.;
RT "Virgibacillus sp. LM2416.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. {ECO:0000256|HAMAP-Rule:MF_02064}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the anti-sigma-I factor
CC RsgI. {ECO:0000256|HAMAP-Rule:MF_02064}.
CC -!- SUBUNIT: Interacts with RsgI. {ECO:0000256|HAMAP-Rule:MF_02064}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_02064}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. SigI subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_02064}.
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DR EMBL; CP022315; ASK60911.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A220TYZ4; -.
DR KEGG; vil:CFK37_01135; -.
DR OrthoDB; 3190733at2; -.
DR Proteomes; UP000198312; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.1740.10; -; 1.
DR HAMAP; MF_02064; Sigma70_SigI; 1.
DR InterPro; IPR014244; RNA_pol_sigma-I.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR NCBIfam; TIGR02895; spore_sigI; 1.
DR PANTHER; PTHR30385:SF6; RNA POLYMERASE SIGMA FACTOR SIGI; 1.
DR PANTHER; PTHR30385; SIGMA FACTOR F FLAGELLAR; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR PIRSF; PIRSF038953; SigI; 1.
DR SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_02064};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_02064}; Reference proteome {ECO:0000313|Proteomes:UP000198312};
KW Sigma factor {ECO:0000256|HAMAP-Rule:MF_02064};
KW Stress response {ECO:0000256|HAMAP-Rule:MF_02064};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_02064};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_02064}.
FT DOMAIN 34..97
FT /note="RNA polymerase sigma-70 region 2"
FT /evidence="ECO:0000259|Pfam:PF04542"
FT DNA_BIND 199..218
FT /note="H-T-H motif"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02064"
FT MOTIF 56..69
FT /note="Polymerase core binding"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02064"
SQ SEQUENCE 243 AA; 28506 MW; 102FCB4BB7E49AE7 CRC64;
MISNQSLKER PLDEMIASAK EGDKDVQNYL LKMYQPYIAK CVSEVCKRYI DPKRDDEFSV
GLFAFNEAIQ TYSSEKGSSF LSFAKLVVKR KVIDYIRFVQ KTPSTASLDE SYDEEKMENP
AEIVAVNERY QEEQEAWYRR EEIADFKDKL SEYKLTLSEL TEASPKHRDA RESAIRTAKT
LYDDAELREY VHQKKKLPIK DLVKRVNVSK KTLERNRKYI LAIFIVLSED YLYLKDYLKG
VAQ
//
