ID A0A221NX95_9ACTN Unreviewed; 377 AA.
AC A0A221NX95;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Propionate 3-nitronate monooxygenase {ECO:0000256|ARBA:ARBA00031155};
GN ORFNames=LK07_10585 {ECO:0000313|EMBL:ASN24418.1};
OS Streptomyces pluripotens.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1355015 {ECO:0000313|EMBL:ASN24418.1, ECO:0000313|Proteomes:UP000031501};
RN [1] {ECO:0000313|EMBL:ASN24418.1, ECO:0000313|Proteomes:UP000031501}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MUSC 137 {ECO:0000313|EMBL:ASN24418.1,
RC ECO:0000313|Proteomes:UP000031501};
RA Ser H.-L., Lee L.-H.;
RT "Genome sequence of Streptomyces pluripotens MUSC 137T.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + 3 O2 + 3 propionate 3-nitronate = 3 3-oxopropanoate + 3
CC H(+) + H2O2 + 2 nitrate + nitrite; Xref=Rhea:RHEA:57332,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632,
CC ChEBI:CHEBI:33190, ChEBI:CHEBI:136067;
CC Evidence={ECO:0000256|ARBA:ARBA00000219};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SIMILARITY: Belongs to the nitronate monooxygenase family. NMO class I
CC subfamily. {ECO:0000256|ARBA:ARBA00009881}.
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DR EMBL; CP022433; ASN24418.1; -; Genomic_DNA.
DR RefSeq; WP_039654622.1; NZ_CP022433.1.
DR AlphaFoldDB; A0A221NX95; -.
DR STRING; 1355015.LK06_009470; -.
DR KEGG; splu:LK06_009470; -.
DR OrthoDB; 9778912at2; -.
DR Proteomes; UP000031501; Chromosome.
DR GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR CDD; cd04730; NPD_like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR004136; NMO.
DR PANTHER; PTHR42747; NITRONATE MONOOXYGENASE-RELATED; 1.
DR PANTHER; PTHR42747:SF3; NITRONATE MONOOXYGENASE-RELATED; 1.
DR Pfam; PF03060; NMO; 1.
DR SUPFAM; SSF51412; Inosine monophosphate dehydrogenase (IMPDH); 1.
PE 3: Inferred from homology;
KW Detoxification {ECO:0000256|ARBA:ARBA00022575};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033, ECO:0000313|EMBL:ASN24418.1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000031501}.
FT DOMAIN 3..357
FT /note="Nitronate monooxygenase"
FT /evidence="ECO:0000259|Pfam:PF03060"
SQ SEQUENCE 377 AA; 38804 MW; 34FC83BA024E9DEA CRC64;
MSSPLTGLFP HPIVQAPMAG GVSVPQLAAA VSEAGGLGFL AAGYKTADGM YQEIKQLRGL
TGRPFGVNVF MPQPEYPGAD ASTTGAAAAA PPDAGAVEIY AHQLAGEAAW YETELGDPDS
GRDDGYDAKL AVLLDDPVPV VSFHFGVPGR EALESLRRAG TFTLVTATTV EEAREVERAG
ADAVIAQGIE AGGHQGTHRD LPEHDGAGIG LLSLIAQIRE AVSIPVVAAG GIMRGSQIAA
VLAAGASAAQ LGTAFLATSE SGAQPLHKQA LTNPLFTHTE LTRAFSGRPA RGLVNRFLRE
HGPYAPAAYP EIHHLTSPLR KAAAKAGDAQ GMALWAGQGH RLARDLPAGE LVRVLAAELA
HARTALSDLP GTGGDQR
//
