ID A0A223P3I5_9SPHI Unreviewed; 1222 AA.
AC A0A223P3I5;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=protein-glutamate O-methyltransferase {ECO:0000256|ARBA:ARBA00012534};
DE EC=2.1.1.80 {ECO:0000256|ARBA:ARBA00012534};
GN ORFNames=MuYL_4727 {ECO:0000313|EMBL:ASU36610.1};
OS Mucilaginibacter xinganensis.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Mucilaginibacter.
OX NCBI_TaxID=1234841 {ECO:0000313|EMBL:ASU36610.1, ECO:0000313|Proteomes:UP000215002};
RN [1] {ECO:0000313|EMBL:ASU36610.1, ECO:0000313|Proteomes:UP000215002}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BJC16-A31 {ECO:0000313|EMBL:ASU36610.1,
RC ECO:0000313|Proteomes:UP000215002};
RG Henan University of Science and Technology;
RA You X.;
RT "Complete genome sequence of Mucilaginibacter sp. strain BJC16-A31.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-
CC glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:24452, Rhea:RHEA-COMP:10208, Rhea:RHEA-COMP:10311,
CC ChEBI:CHEBI:29973, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:82795; EC=2.1.1.80;
CC Evidence={ECO:0000256|ARBA:ARBA00001541};
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DR EMBL; CP022743; ASU36610.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A223P3I5; -.
DR KEGG; muc:MuYL_4727; -.
DR OrthoDB; 9813151at2; -.
DR Proteomes; UP000215002; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0008984; F:protein-glutamate methylesterase activity; IEA:InterPro.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:InterPro.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-UniRule.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd16434; CheB-CheR_fusion; 1.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 1.10.287.130; -; 1.
DR Gene3D; 1.10.155.10; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR Gene3D; 3.40.50.180; Methylesterase CheB, C-terminal domain; 1.
DR Gene3D; 3.30.450.20; PAS domain; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR035909; CheB_C.
DR InterPro; IPR022642; CheR_C.
DR InterPro; IPR000780; CheR_MeTrfase.
DR InterPro; IPR022641; CheR_N.
DR InterPro; IPR036804; CheR_N_sf.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013655; PAS_fold_3.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR000673; Sig_transdc_resp-reg_Me-estase.
DR PANTHER; PTHR24422; CHEMOTAXIS PROTEIN METHYLTRANSFERASE; 1.
DR PANTHER; PTHR24422:SF25; CHEMOTAXIS PROTEIN METHYLTRANSFERASE; 1.
DR Pfam; PF01339; CheB_methylest; 1.
DR Pfam; PF01739; CheR; 1.
DR Pfam; PF03705; CheR_N; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF08447; PAS_3; 1.
DR PRINTS; PR00996; CHERMTFRASE.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00138; MeTrc; 1.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF47757; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR SUPFAM; SSF47384; Homodimeric domain of signal transducing histidine kinase; 1.
DR SUPFAM; SSF52738; Methylesterase CheB, C-terminal domain; 1.
DR SUPFAM; SSF55785; PYP-like sensor domain (PAS domain); 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS50122; CHEB; 1.
DR PROSITE; PS50123; CHER; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50113; PAC; 1.
DR PROSITE; PS50112; PAS; 1.
PE 4: Predicted;
KW Chemotaxis {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000215002};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..196
FT /note="CheB-type methylesterase"
FT /evidence="ECO:0000259|PROSITE:PS50122"
FT DOMAIN 210..482
FT /note="CheR-type methyltransferase"
FT /evidence="ECO:0000259|PROSITE:PS50123"
FT DOMAIN 871..942
FT /note="PAS"
FT /evidence="ECO:0000259|PROSITE:PS50112"
FT DOMAIN 947..1000
FT /note="PAC"
FT /evidence="ECO:0000259|PROSITE:PS50113"
FT DOMAIN 1011..1222
FT /note="Histidine kinase"
FT /evidence="ECO:0000259|PROSITE:PS50109"
FT COILED 650..737
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 854..881
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 26
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 53
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 143
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
SQ SEQUENCE 1222 AA; 137998 MW; CF3B6E972E8D44C4 CRC64;
MPLPNLVDKD LVLSDNQFPV VGIGASAGGI EAFKLFLQSV PEKSGMAYVF VQHLSPDYNS
YLPEIFKKST KIPVLLIADN IHLEPDHVYI IPPGYILTAT DGILKLEPIK NKKVKTIDIF
FSSLAVVHQS FAIGIVLSGA LDDGTVGLQV IKAYGGLTFA QSEDSATFDS MPRSAIRSGA
VDFILPAEQI MSKLISINQP FNDGTIEGQI GKNEPEHDEE IFKQLLTVLR IRRGVDFVNY
KQSTIKRRIV RRMALVKIEK PKEYLNFLRE NKPEQDALYN DMLISVTNFF RDPQSFEVLC
ESILPAILIH KTPNEAIRIW VAGCATGEEA YSIAICLQEY LGDKASARKI QIFATDVSEI
AIARARTGIY RQNDLGGLST LQIQQFFNKL DGSYQVNKTI RDMCVFAHHN LLKDPPFSRI
DLVSCRNVMI YLEPVLQKRA LSTFHYALNE RGYLMLGKSE TIGTSTDIFT HVNKTQKIYQ
SKGPHGRVRA VTSERSEQTL KAIDQIIPEN SSENDINKVA DALLLSKYTP AGVMVNQSFD
ITQFRGKTDP WLAVSPGKPS FNVLKMAREG LSFEIRSLLH LAKTKQEAMR KEGISFKIDN
ERHYVNIEVV PLSDTEDNYY LILFQNSMLS EPSSLTDSSK LNSRHTPENI NAWVQQIDQL
EKELTQTRED MRSITETQEA ANEELQSANE ELLSGSEELR SLNEELETST EELQSSNEEI
TIVNNELLDR NEQLNNSRKY TEEIFNTIHD PLVILDKDLV VLRATDGFYN MFRVNEEDTE
GKFIYDLGNK QWDIPALREQ LEKTLPQQGF FKAFEVDHIF STIGRRIMQL TARQFDTYTD
EKLTLLAIHD ITDKRKVEEG LAEAERLLAE SKERLHFAIE SAGIGAWDFN PITKELIWDN
RCKELYGLAP ADSIDYAGFL SYIHTDDRSA TDNSINKTLK GANRGEFNEE YRTIGKNDQK
LRWIKSKGKA YFDKDNKATR FIGTVLDVSI EKDAEQSTIE MLRKKDEFIS IASHELKTPV
TSLKASLQLL DRMKNDPSPM LPRFLDQANR SMLKITHLID ELLNVNSMKE GQLRLNKSVF
VIAGMLKDCC GHVRAAGVHE LVVQGDETLK VDADEDRIEQ VVVNLVNNVV KYAPESKNIY
FIVEKEGNTA KVSVKDTGLG ISADKIPHLF DRYYRADYGG GQYSGMGLGL YISSEIIKRH
GGKIGVQSEV GKGSTFWFTL PL
//
