ID   A0A225VA54_9STRA        Unreviewed;       688 AA.
AC   A0A225VA54;
DT   25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT   25-OCT-2017, sequence version 1.
DT   24-JAN-2024, entry version 23.
DE   RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE            EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
DE   Flags: Fragment;
GN   ORFNames=PHMEG_00026925 {ECO:0000313|EMBL:OWZ01647.1};
OS   Phytophthora megakarya.
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4795 {ECO:0000313|EMBL:OWZ01647.1, ECO:0000313|Proteomes:UP000198211};
RN   [1] {ECO:0000313|Proteomes:UP000198211}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=zdho120 {ECO:0000313|Proteomes:UP000198211};
RA   Ali S., Shao J., Larry D.J., Kronmiller B., Shen D., Strem M.D.,
RA   Melnick R.L., Guiltinan M.J., Tyler B.M., Meinhardt L.W., Bailey B.A.;
RT   "Phytopthora megakarya and P. palmivora, two closely related causual agents
RT   of cacao black pod achieved similar genome size and gene model numbers by
RT   different mechanisms.";
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000256|RuleBase:RU363044};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|RuleBase:RU363044};
CC   -!- SIMILARITY: Belongs to the helicase family.
CC       {ECO:0000256|RuleBase:RU363044}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OWZ01647.1}.
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DR   EMBL; NBNE01006698; OWZ01647.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A225VA54; -.
DR   STRING; 4795.A0A225VA54; -.
DR   EnsemblProtists; OWZ01647; OWZ01647; PHMEG_00026925.
DR   OrthoDB; 994956at2759; -.
DR   Proteomes; UP000198211; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR   CDD; cd18809; SF1_C_RecD; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR010285; DNA_helicase_pif1-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR049163; Pif1-like_2B_dom.
DR   PANTHER; PTHR10492:SF57; ATP-DEPENDENT DNA HELICASE; 1.
DR   PANTHER; PTHR10492; UNCHARACTERIZED; 1.
DR   Pfam; PF05970; PIF1; 1.
DR   Pfam; PF21530; Pif1_2B_dom; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|RuleBase:RU363044};
KW   DNA damage {ECO:0000256|RuleBase:RU363044};
KW   DNA recombination {ECO:0000256|RuleBase:RU363044};
KW   DNA repair {ECO:0000256|RuleBase:RU363044};
KW   Helicase {ECO:0000256|RuleBase:RU363044, ECO:0000313|EMBL:OWZ01647.1};
KW   Hydrolase {ECO:0000256|RuleBase:RU363044};
KW   Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198211}.
FT   DOMAIN          522..568
FT                   /note="DNA helicase Pif1-like 2B"
FT                   /evidence="ECO:0000259|Pfam:PF21530"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:OWZ01647.1"
SQ   SEQUENCE   688 AA;  78017 MW;  8A729231B91304FB CRC64;
     SFEDIRTIND VVHETFHDDA LAAGYLEDDR EWEECLAEAV SFKMPYALRQ LFGTILVYSL
     PDHASALWDR FKNDFLKDYF KSIAERDRQN NIERDEEVRL RMAEYKCLKW VAEYLLSNGK
     TLDMCGLPEL STYEDVSEEV EREDTGPSDV VANEIATYEP EDLARTTALA DQMNENQKKS
     SSKSSMLWTI PLMDRSCGTG KSFLLEQMLA HVRLQKKVAF AVASSGIAAT LLTGGHTAHS
     TFRIPLKLSE FSTCSLPWQS QKAELIRNAS LIIWDEAPMM HRACFEAVDR SFRDIMKNDL
     GPFGGKVMVF SGGHRQILPV LKDATRAETL EACFKSSPLW NHLKKVRLTE NMRVRTAPDP
     ASAAELAEFS EFLLQIGEGR YPVNSDISEN DICLPRDMCI IPEERGHYRW DDEEHKESED
     VEAPPNFNLL PATELPFEVG ETNPDDDRRE RNVNALIDAV YPGVGDENLQ DDFVDRAILA
     PTNASVRRIN EMVAERLTGE TKEYLSVDSL EGVADPNMFE QEFLNSLNFS GIPPHRIVLK
     VGTPIIMIRN LNSDAGLCNG TRLRVVSLRG RSIEATIMSE PFKGKPVFIP RIIFYSEDDD
     KEFPFKLRRK QFPVLPAFAM TINKAQRQSI HQVGIYLESP VFAYGQLYVA LSRVTSRKVI
     KIAVDPSAID DNGNMHTKNI VYREIFEL
//