ID A0A226GNP4_9FLAO Unreviewed; 480 AA.
AC A0A226GNP4;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:OXA83101.1};
GN ORFNames=B0A66_22555 {ECO:0000313|EMBL:OXA83101.1};
OS Flavobacterium hercynium.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=387094 {ECO:0000313|EMBL:OXA83101.1, ECO:0000313|Proteomes:UP000198345};
RN [1] {ECO:0000313|EMBL:OXA83101.1, ECO:0000313|Proteomes:UP000198345}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18292 {ECO:0000313|EMBL:OXA83101.1,
RC ECO:0000313|Proteomes:UP000198345};
RA Stine C., Li C., Tadesse D.;
RT "Whole genomes of Flavobacteriaceae.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OXA83101.1}.
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DR EMBL; MUGW01000092; OXA83101.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A226GNP4; -.
DR OrthoDB; 9806009at2; -.
DR Proteomes; UP000198345; Unassembled WGS sequence.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006046; Alpha_amylase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR PRINTS; PR00110; ALPHAAMYLASE.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000198345}.
FT DOMAIN 71..387
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 260
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 284
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 171
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 235
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 406
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 480 AA; 54431 MW; 9AD1745D522428F6 CRC64;
MKKTHASIYF VAVIAFLFYS CSQNETDSVD AKAESQQFKI INVTNHDGKS FSTGDAKSSE
TGKYVENPGG GVMMQAFYWD VPAGGNWWNT VNGKLTDWSN AGIGSIWLPP ASKAQNGPLS
MGYDPTDYFD FGDYNQNGTI ETRFGSKTEL VNLITKAHAE NMKVYADIVL NHNSGGQSEA
NPFTGTNTWT NFTGIASGKF KRTYNNFYKN SFGNNDEGFF GGFPDLCHAD PYVQDWLWIR
ADGVGKYYKN TMKFDGWRFD YVKGFGPWVV NAWNANVGGF SVGEYWDANV NTLEWWANNA
NSSVFDFACY YKMNDAFDGN NLALLNDDMM WKRNPFKAVT FVTNHDTDEI SSKLLAYSYI
LTHEGYPTLF YKDYEETLDK NKLNNLIWIH NNKATGNTSI LYSDNDEYVA RRNGYNGNPG
LVVYINNSTS WQERWVETNW ASTQIKDFTG NSSWYPTTQG DKWVKIQCPP KGYTVWSVNQ
//
