ID A0A226H6W9_9FLAO Unreviewed; 686 AA.
AC A0A226H6W9;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=Ricin B lectin domain-containing protein {ECO:0000259|SMART:SM00458};
GN ORFNames=B0A66_13740 {ECO:0000313|EMBL:OXA90059.1};
OS Flavobacterium hercynium.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=387094 {ECO:0000313|EMBL:OXA90059.1, ECO:0000313|Proteomes:UP000198345};
RN [1] {ECO:0000313|EMBL:OXA90059.1, ECO:0000313|Proteomes:UP000198345}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18292 {ECO:0000313|EMBL:OXA90059.1,
RC ECO:0000313|Proteomes:UP000198345};
RA Stine C., Li C., Tadesse D.;
RT "Whole genomes of Flavobacteriaceae.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OXA90059.1}.
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DR EMBL; MUGW01000026; OXA90059.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A226H6W9; -.
DR Proteomes; UP000198345; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd09004; GH43_bXyl-like; 1.
DR CDD; cd00161; RICIN; 2.
DR Gene3D; 2.80.10.50; -; 6.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR InterPro; IPR026444; Secre_tail.
DR NCBIfam; TIGR04183; Por_Secre_tail; 1.
DR PANTHER; PTHR43772:SF2; BETA-1,4-XYLOSIDASE (EUROFUNG); 1.
DR PANTHER; PTHR43772; ENDO-1,4-BETA-XYLANASE; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR Pfam; PF18962; Por_Secre_tail; 1.
DR Pfam; PF14200; RicinB_lectin_2; 3.
DR SMART; SM00458; RICIN; 2.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 2.
DR PROSITE; PS50231; RICIN_B_LECTIN; 2.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00022651};
KW Reference proteome {ECO:0000313|Proteomes:UP000198345};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Xylan degradation {ECO:0000256|ARBA:ARBA00022651}.
FT DOMAIN 304..441
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT DOMAIN 450..587
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT ACT_SITE 23
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 178
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 129
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 686 AA; 76534 MW; 929891AA5EBA9B9D CRC64;
MLIGIVIPAH AQLSGNPLFT GADPEIHYFN NKYYIYTTAI YGTQFHAYSS NDLTNWHDEG
LIFDLFPDSP WAQYNGWAPA VVFRNNKYYF YYTAETKIGL AVGNTPIGPF TDIGAPLIAT
DPYTDDIIDA NVFIDDDGQA YIYYGGSGKS RMVVRKLNAD MISLATGPTD ITPQNYTEGP
YMVKRKGIYY MMYSNGAWFN DSYNVQYSTS NSPMGPWTYR GKVLSSNAED KGPGHHGVLK
IGNCDEYYMV YHRYENGTSG ERKIAIDRMY FNSNDLIEPV NMTNYGVAPR VPDQSCPTQN
ITSGGIYKLT HKGTNQCLDV VNNASQSGIN VQQSTDNGND AQRWVITLEA DGYYKLTHKG
TNQCLDVANN SNQPGANVQQ WVDNNNDAQR WKIELMSDGY CKLTHKGTNQ CLDVKDNSNQ
SGANVQQWTD LGTDAQRWKL DLIEVPIVSG GVYKLTHKGT NQCLDVSDGS TQQGANVQQY
TGNDTDAQRW VITKESDGFY KLTHKGTNQC LDVDNNSSQP GANVQQWSDL GTDAQRWKIE
LMSDGYFKLT HKGTNQCLDV ANNSAEPGTN VHQWTDNNND AQRWKLDLIK PVTPSSTAKS
SKSIVGNQDE ALANTVTIYP NPVKNRLFIN SEIKDMKISI FSQTGSLVKV QRNIDNSVDV
SGLTSGIYFA VFEKDNTKIT KQFIKE
//
