ID A0A226HX61_9FLAO Unreviewed; 619 AA.
AC A0A226HX61;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=B0A75_15170 {ECO:0000313|EMBL:OXA98010.1};
OS Flavobacterium oncorhynchi.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=728056 {ECO:0000313|EMBL:OXA98010.1, ECO:0000313|Proteomes:UP000198336};
RN [1] {ECO:0000313|EMBL:OXA98010.1, ECO:0000313|Proteomes:UP000198336}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 59446 {ECO:0000313|EMBL:OXA98010.1,
RC ECO:0000313|Proteomes:UP000198336};
RA Stine C., Li C., Tadesse D.;
RT "Whole genomes of Flavobacteriaceae.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OXA98010.1}.
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DR EMBL; MUHA01000024; OXA98010.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A226HX61; -.
DR Proteomes; UP000198336; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..619
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5013370830"
FT DOMAIN 33..356
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 398..508
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 528..586
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 181
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 265
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 619 AA; 70503 MW; 47B333F3D831E7FB CRC64;
MKKVYYTLLF AFFTLLSASA QQKQTFAISG GDFLLNGKPI QIHSGEMHYS RIPQQYWRHR
LKMMKAMGLN AVATYVFWNY HETAPGVWDF KTGNKNLAEY IKTAQEEGLF VILRPGPYVC
AEWEFGGYPF FLQNVPNMVI RGNNKEYLAA TKAYFTQLYN QVKDLQITKG GPIIMVQGEN
EFGSYVAQRK DVPLEEHKKY SAAVFQQLKD LGFEVPFFTS DGSWLFEGGA LPGALPTANG
ESDIAKLKEV VNKFNNGQGP YMVAEFYPGW LDHWAEPFPT QKPEQTARQT KKYLDNKVSF
NYYMVHGGTN FGFTSGANYD KEHDIQPDMT SYDYDAPISE AGWATPKYNM LRELLKTSET
PAIPEKMPII EIPKIALTKT VDLADLKAKI SPVYEDTPQT FEQLNQGDGY VWYSKKFTQP
ISGKLELKGL RDYAIVYVNG KKVAELNRYY KKYDCEIEVP FNATLDIVVE NMGRINYGSQ
INENNKGIIS PVIINGQTIT GDWEMYKLPM SQEPDLTAYK NSGKVNRPTL YQGTFTLNKT
GDTFLDMRDW GKGIVFVNGI NIGRYWSVGP QQTLYLPGCW LKKGKNNIVI FEQKNETLQK
ELKSITTPIL EDLRPEKGQ
//
