ID   A0A229UZF4_9BACL        Unreviewed;       243 AA.
AC   A0A229UZF4;
DT   25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT   25-OCT-2017, sequence version 1.
DT   24-JAN-2024, entry version 16.
DE   SubName: Full=Aminoglycoside phosphotransferase {ECO:0000313|EMBL:OXM88349.1};
GN   ORFNames=CF651_00330 {ECO:0000313|EMBL:OXM88349.1};
OS   Paenibacillus rigui.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=554312 {ECO:0000313|EMBL:OXM88349.1, ECO:0000313|Proteomes:UP000215509};
RN   [1] {ECO:0000313|EMBL:OXM88349.1, ECO:0000313|Proteomes:UP000215509}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 16352 {ECO:0000313|EMBL:OXM88349.1,
RC   ECO:0000313|Proteomes:UP000215509};
RA   Mayilraj S.;
RT   "Genome sequencing and assembly of Paenibacillus rigui.";
RL   Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000256|ARBA:ARBA00006219}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OXM88349.1}.
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DR   EMBL; NMQW01000001; OXM88349.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A229UZF4; -.
DR   OrthoDB; 3806873at2; -.
DR   Proteomes; UP000215509; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   Gene3D; 3.90.1200.10; -; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251};
KW   Magnesium {ECO:0000256|PIRSR:PIRSR000706-2};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR000706-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000215509};
KW   Transferase {ECO:0000313|EMBL:OXM88349.1}.
FT   DOMAIN          39..225
FT                   /note="Aminoglycoside phosphotransferase"
FT                   /evidence="ECO:0000259|Pfam:PF01636"
FT   ACT_SITE        167
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000706-1"
FT   BINDING         172
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000706-2"
FT   BINDING         185
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000706-2"
SQ   SEQUENCE   243 AA;  27860 MW;  298C3103BA096A84 CRC64;
     MKRTEVSFDI ETIPLTIRQF INGATIYDSS CSEHAKTLFV SGSERAFLKI SKRGTLEREY
     HMTKFLHQHN VAPNAIAYES DLDYDYLFTE AVSGEDGTAE LHLENPRKLA CVFGEYLKML
     HSLPIEGCPY GNRTHELLND AQINGIDLNM LNEFSYSAID NVVIHGDYCL PNIIMDHFSF
     KGFIDLGYGG VGDRHYDIYW GIWTLNYNLK TDTYRDIFLD AYGRSNIDDD GLNYFTKLNK
     LTD
//