ID A0A232F9W8_9HYME Unreviewed; 1815 AA.
AC A0A232F9W8;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|RuleBase:RU366018};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU366018};
GN ORFNames=TSAR_014521 {ECO:0000313|EMBL:OXU27651.1};
OS Trichomalopsis sarcophagae.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Proctotrupomorpha;
OC Chalcidoidea; Pteromalidae; Pteromalinae; Trichomalopsis.
OX NCBI_TaxID=543379 {ECO:0000313|EMBL:OXU27651.1, ECO:0000313|Proteomes:UP000215335};
RN [1] {ECO:0000313|EMBL:OXU27651.1, ECO:0000313|Proteomes:UP000215335}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Alberta {ECO:0000313|EMBL:OXU27651.1,
RC ECO:0000313|Proteomes:UP000215335};
RC TISSUE=Whole body {ECO:0000313|EMBL:OXU27651.1};
RX PubMed=28648823; DOI=10.1016/j.cub.2017.05.032;
RA Martinson E.O., Mrinalini, Kelkar Y.D., Chang C.H., Werren J.H.;
RT "The Evolution of Venom by Co-option of Single-Copy Genes.";
RL Curr. Biol. 27:2007-2013(2017).
CC -!- FUNCTION: Ubiquitin ligase protein which is a component of the N-end
CC rule pathway. Recognizes and binds to proteins bearing specific N-
CC terminal residues that are destabilizing according to the N-end rule,
CC leading to their ubiquitination and subsequent degradation.
CC {ECO:0000256|RuleBase:RU366018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU366018};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU366018}.
CC -!- SIMILARITY: Belongs to the UBR1 family. {ECO:0000256|ARBA:ARBA00009750,
CC ECO:0000256|RuleBase:RU366018}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OXU27651.1}.
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DR EMBL; NNAY01000561; OXU27651.1; -; Genomic_DNA.
DR STRING; 543379.A0A232F9W8; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000215335; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR GO; GO:0071596; P:ubiquitin-dependent protein catabolic process via the N-end rule pathway; IEA:UniProtKB-UniRule.
DR CDD; cd19672; UBR-box_UBR1_like; 1.
DR Gene3D; 2.10.110.30; -; 1.
DR Gene3D; 3.30.1390.10; -; 1.
DR Gene3D; 1.10.10.2670; E3 ubiquitin-protein ligase; 1.
DR InterPro; IPR003769; ClpS_core.
DR InterPro; IPR042065; E3_ELL-like.
DR InterPro; IPR044046; E3_ligase_UBR-like_C.
DR InterPro; IPR014719; Ribosomal_bL12_C/ClpS-like.
DR InterPro; IPR039164; UBR1-like.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR003126; Znf_UBR.
DR PANTHER; PTHR21497:SF24; E3 UBIQUITIN-PROTEIN LIGASE UBR1; 1.
DR PANTHER; PTHR21497; UBIQUITIN LIGASE E3 ALPHA-RELATED; 1.
DR Pfam; PF02617; ClpS; 1.
DR Pfam; PF18995; PRT6_C; 1.
DR Pfam; PF02207; zf-UBR; 1.
DR SMART; SM00396; ZnF_UBR1; 1.
DR SUPFAM; SSF54736; ClpS-like; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51157; ZF_UBR; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU366018};
KW Reference proteome {ECO:0000313|Proteomes:UP000215335};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU366018};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU366018};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366018};
KW Zinc-finger {ECO:0000256|RuleBase:RU366018}.
FT DOMAIN 112..183
FT /note="UBR-type"
FT /evidence="ECO:0000259|PROSITE:PS51157"
FT ZN_FING 112..183
FT /note="UBR-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00508"
FT REGION 1002..1029
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1815 AA; 205420 MW; A02330C10DF282A7 CRC64;
MSNESDDTEG HGINDVELSL TMPIIQNTCP PCVSIWMEKM RQGVLSNTHF KEHWRVWVPK
IYKLEPTDNC LEWVFNEKEA QTILYDPLEE FICNGNPHEI LKQLSQNDRP PSICGRVFKA
GEPTYSCREC GMDSTCVLCV DCFKQSAHKN HKYKMGISIG GGCCDCGDTE AWKKEPFCKI
HLAGIESKDA PSNKLPDDMA QRAAATFRAV LKYCYDLLSM EHSPSLPNDL CIKNDEDSVS
LLASNDTYCT VLFNDETHTF DQVIITLTRV IKCSQRDAIE YVTNIDREGR AVVKCSGFQH
CNELKSEIEK FTSRHNSRPL KVLVEHAHVV AHQIFAMKLL GWLQQFISHC EGFRLIFSDV
ALNDKMPDIP IVKGILIRDY QLWKAARTCW HRLFISGMLT EYESKKALAI DFTNNYGTVL
KDFIRDDHDH SYSIASLAVQ LFTVPTLAHH LIAHHDALLI LFNTFISESS RRCNAAGKLE
FERNAPNNAF KRAQYILYDL RYLLSAKPET WTDELRRGFL QGVSLLLQLL GSMQWMDAVV
RQVGQHMEYE QEWESAFNLY IKLSPVISLA LEWCGSDRIV LIKAFRLVLK KLEEQPGKAP
TNFREVADHS ASCIHYDVST EPVSIHLPLS RFLAGLFLHL EKYDLTFQGT EFQTLSKPTP
EEIIEPVLRA QAMISQVHAG MWRRNGYSLI HQLYFYHNVK CRTEMLDKDI VLLQAGAALI
ESNEFLIHIL NKFNLINWAQ PDFETALLKT CEEDSIRQTI NLVEEFLALL ITIIGERYVP
GVGRVTQDDC LKREIIQQLC IKPLSHSELN KTLPDDVHHE TGMERVIHEV ADFKKPQVVS
AGKGVYELKF NFYSEYNVFF YHYTKEELSK SEEAQRKRRK TVGELECCPP PKLPRLTEML
SLVTNLLQCD VMLSIMNIVL TRTLDLKARS FSEPQVHKIL HLIGYALQEQ ESGYYPFLAF
TERAIKWDIY KLLETMQNSP RIEAHKDLLT WVLEKYRQVA GSPIPEAPEP PAPQPTDPGE
DTEKLEKGSQ EWRMKMAALK RAQVMAQMEA MQKHFMKKNA KMFQEAALDV SNKGSNDRGS
AMDLSESLDE TSVALGRRQT NRLCFEKSYT CILCQEDQCI KADESAMVLA AFVQQSTVLC
QLPHERQKAQ QPPQSRSVAE KTPLFLSSYL GASPHTSTCG HVMHSSCWQK HFDNVLAKEN
RRPYRLRQPA SFDVEKHEYL CPLCECLSNT VLPVLPSLGT LQPTPADQPD LSFDRWLDIM
SALTKCKRSN DNKDTPKVAR NEDCRYCKFL SQQELDAAEA ESGGNAQATP STSSLISCPI
EAIKAQGPDG EALLSLYSQP GPRLSKNLVE MIQLFTQTTY TKGLGCEPHD DDSRVPLLAW
KSTAYTVHAI EFLLRDTEKP LLGALSSRQR DSLEGLARIS AVISATWPSN VAGSTSPTSS
ENIAEYALEC LTCFLNNAES NYSILSWDPF GMLVTLLASL SNLLSSRTDD PHSIITGNVA
EAHATRLVFT ALVVKVLLMS NFDQDEKMDV DDDVADDNNE AEMRDASKPS AILNVMKALN
ISTGNKSDAE IWLKVQECCE PFLRCCVLYF HYVTDVPAPV DLTVLGGDSF EAMCNYLGLP
ETFEELFNTE KVLQLAVQWS KHPEMVNKDT ATREPLKINR LVDLPDDYSE LMNTVSMFSC
PNSDKEDSLN PTMCLVCGGM LCSQSYCCQV ELNKIMVGAC TYHAHECGAG VGVFLRVRDC
EIVFLRSPNR GSYGCPPYFD EYGETDQGLH RGNPLKLCRD KYKRLNQMWL GHGLYECIAR
AIETATSATG QWQHL
//
