ID A0A238LB02_9RHOB Unreviewed; 558 AA.
AC A0A238LB02;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543,
GN ECO:0000313|EMBL:SMY06605.1};
GN ORFNames=LOM8899_00732 {ECO:0000313|EMBL:SMY06605.1};
OS Flavimaricola marinus.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Paracoccaceae; Flavimaricola.
OX NCBI_TaxID=1819565 {ECO:0000313|EMBL:SMY06605.1, ECO:0000313|Proteomes:UP000201613};
RN [1] {ECO:0000313|EMBL:SMY06605.1, ECO:0000313|Proteomes:UP000201613}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CECT 8899 {ECO:0000313|EMBL:SMY06605.1,
RC ECO:0000313|Proteomes:UP000201613};
RA Song R., Chenine A.L., Ruprecht R.M.;
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; FXZK01000001; SMY06605.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A238LB02; -.
DR OrthoDB; 9761733at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000201613; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:SMY06605.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000201613}.
FT BINDING 67..74
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 558 AA; 59736 MW; 6E3B76A930017B1E CRC64;
MTYKTDIEIA RDAQKRPIQE IGAKLGIGAE DLLPYGHDKA KVSQSFINSV QDRPNGKLVL
VTAINPTPAG EGKTTTTVGL GDGLNRIGKK AAICIREASL GPNFGMKGGA AGGGLAQVVP
MEDMNLHFTG DFHAITSAHN LLSAMIDNHI YWGNELEIDI RRIVWRRVLD MNDRALRQIT
ASLGGVANGF PRETGFDITV ASEVMAILCL ARDLADLQER LGNMIVAYRR DRSPVFCRDI
KADGAMTVLL KDAMQPNLVQ TLENNPAFVH GGPFANIAHG CNSVTATTTA LKIADYVVTE
AGFGADLGAE KFMNIKCRKA GLAPDAVVLV ATVRAMKMNG GVAKADLGEE NVAAVEAGCP
NLGRHLANLK SFGVPVVVAI NHFVTDTEAE VKAVQDYIAA QGAEAIECRH WAMGSEGIED
LARKVVELAE GGTSAFAPLY PDDMPLMKKV ETIAKRIYHA DAVLADKSVR NQLAAWEEQG
YGHLPICMAK TQYSFSTDPN LRGAPTGHSV PIREVRLSAG AGFVVVICGE IMTMPGLPRI
PSAESIRLND DGQIEGLF
//
