ID A0A239QQK2_9MICO Unreviewed; 543 AA.
AC A0A239QQK2;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=SAMN06298212_102182 {ECO:0000313|EMBL:SNU00649.1};
OS Ruaniaceae bacterium KH17.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Ruaniaceae.
OX NCBI_TaxID=1945888 {ECO:0000313|EMBL:SNU00649.1, ECO:0000313|Proteomes:UP000214750};
RN [1] {ECO:0000313|EMBL:SNU00649.1, ECO:0000313|Proteomes:UP000214750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KH17 {ECO:0000313|EMBL:SNU00649.1,
RC ECO:0000313|Proteomes:UP000214750};
RA Sun Z.S., Albrecht U., Echele G., Lee C.C.;
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; FZQV01000002; SNU00649.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A239QQK2; -.
DR OrthoDB; 9761733at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000214750; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:SNU00649.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000214750}.
FT BINDING 49..56
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 543 AA; 56924 MW; 5C49DACDA38443B6 CRC64;
MSIPIVEIAH SAGIDADSLI SYGRDIAKCP LTPTSGEAPL VMVTAITPTP LGEGKTTTVV
GLVQGLARLG RRPIATLRQP SLGPTFGIKG GAAGAGASQV IPAEKLNLHL TGDFHAITAA
HNLLSAMVDN HLHHGNTLGI EPHSITWPRV LDVNDRALRN IVVGLGRQLD GVPRQSSFDI
TAASEIMTII ALSTSLEDLR ERLGRIVVGY THHGVPVTAE QLQAAGAMTA LLRDALMPNL
MQTTEGVPVL IHAGPFGNIS IGNSSVVADL VASSLGDIVV TESGFGADLG AERMVNLKSR
VSGYAPSAAV VVVTVRAMKA YSGAHYIVPG RPLPPAMLEE NPAQVAAGLA NLAHHLRIVG
QWGIPTVVAI NAFPEDHESE HSVIAEFCAE RGVRCAVSRH VAEGGAGAED LAREVIEAVS
GPVEVRRDYE PEQSLIEKME AVARNVYGAA GIDLAPTARR ELARFTERGF GGLHVVLAKT
HLHIAHTAHP GEGWLLPIRE VRLAAGAGYV YAITGSLSTM PGLPSHPNAE KIDVVDGEII
GLT
//
