ID   A0A240CAP7_SERFI        Unreviewed;       288 AA.
AC   A0A240CAP7;
DT   25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT   25-OCT-2017, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU003887};
DE            EC=5.4.99.- {ECO:0000256|RuleBase:RU003887};
GN   Name=rluF_2 {ECO:0000313|EMBL:SNW04929.1};
GN   ORFNames=SAMEA4384070_04086 {ECO:0000313|EMBL:SNW04929.1};
OS   Serratia ficaria.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia.
OX   NCBI_TaxID=61651 {ECO:0000313|EMBL:SNW04929.1, ECO:0000313|Proteomes:UP000215134};
RN   [1] {ECO:0000313|EMBL:SNW04929.1, ECO:0000313|Proteomes:UP000215134}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC12148 {ECO:0000313|EMBL:SNW04929.1,
RC   ECO:0000313|Proteomes:UP000215134};
RG   Pathogen Informatics;
RL   Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2604) in 23S rRNA = pseudouridine(2604) in 23S rRNA;
CC         Xref=Rhea:RHEA:38875, Rhea:RHEA-COMP:10093, Rhea:RHEA-COMP:10094,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.21;
CC         Evidence={ECO:0000256|ARBA:ARBA00036535};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(35) in tRNA(Tyr) = pseudouridine(35) in tRNA(Tyr);
CC         Xref=Rhea:RHEA:60556, Rhea:RHEA-COMP:15607, Rhea:RHEA-COMP:15608,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000256|ARBA:ARBA00036390};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000256|ARBA:ARBA00008348, ECO:0000256|RuleBase:RU003887}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LT906479; SNW04929.1; -; Genomic_DNA.
DR   RefSeq; WP_061798669.1; NZ_QTUE01000001.1.
DR   AlphaFoldDB; A0A240CAP7; -.
DR   STRING; 1411141.GCA_001590885_03397; -.
DR   GeneID; 75029208; -.
DR   KEGG; sfj:SAMEA4384070_4086; -.
DR   OrthoDB; 9807213at2; -.
DR   Proteomes; UP000215134; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02554; PseudoU_synth_RluF; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR   PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR   PANTHER; PTHR47683:SF2; S4 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003887};
KW   Reference proteome {ECO:0000313|Proteomes:UP000215134};
KW   RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT   DOMAIN          7..63
FT                   /note="RNA-binding S4"
FT                   /evidence="ECO:0000259|SMART:SM00363"
FT   REGION          238..288
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   288 AA;  32019 MW;  5F694BF533EA26CA CRC64;
     MLTKPSTRLN KYISESGICS RRDADRYIEQ GNVFINGKRV ALGDRVFPGD VVKVNGQLIE
     PRNEDDLVFI ALNKPVGIVS TTEDGEKDNI VDFVNHSTRI FPIGRLDKDS QGLIFLTNHG
     DLVNKILRAG NDHEKEYRVT VNKPVTDEFI RGLGAGVPML GTVTKKCKVK KEAPFVFRIV
     LVQGLNRQIR RMCEHFGYEV TKLERTRIMN VSLTGLPPGE WRDLTDDELI ELFKSIEDSS
     SEAKPAKKAQ PAARKKPAGS GPKSAEKPGG APAGRKRFAQ PGRKKKGR
//