ID A0A240UMD4_9GAMM Unreviewed; 443 AA.
AC A0A240UMD4;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE EC=3.1.11.6 {ECO:0000256|HAMAP-Rule:MF_00378};
DE AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE Short=Exonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
GN Name=xseA {ECO:0000256|HAMAP-Rule:MF_00378};
GN ORFNames=B9H00_03695 {ECO:0000313|EMBL:ART62286.1}, C8D96_0859
GN {ECO:0000313|EMBL:RKD87387.1};
OS Kushneria marisflavi.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Kushneria.
OX NCBI_TaxID=157779 {ECO:0000313|EMBL:ART62286.1, ECO:0000313|Proteomes:UP000194457};
RN [1] {ECO:0000313|EMBL:ART62286.1, ECO:0000313|Proteomes:UP000194457}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SW32 {ECO:0000313|EMBL:ART62286.1};
RA Song R., Chenine A.L., Ruprecht R.M.;
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RKD87387.1, ECO:0000313|Proteomes:UP000285584}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15357 {ECO:0000313|EMBL:RKD87387.1,
RC ECO:0000313|Proteomes:UP000285584};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC insoluble oligonucleotides, which are then degraded further into small
CC acid-soluble oligonucleotides. {ECO:0000256|HAMAP-Rule:MF_00378}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00378,
CC ECO:0000256|RuleBase:RU004355};
CC -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00378}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00378,
CC ECO:0000256|RuleBase:RU004355}.
CC -!- SIMILARITY: Belongs to the XseA family. {ECO:0000256|HAMAP-
CC Rule:MF_00378, ECO:0000256|RuleBase:RU004355}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP021358; ART62286.1; -; Genomic_DNA.
DR EMBL; RAPM01000001; RKD87387.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A240UMD4; -.
DR KEGG; kma:B9H00_03695; -.
DR OrthoDB; 9802795at2; -.
DR Proteomes; UP000194457; Chromosome.
DR Proteomes; UP000285584; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd04489; ExoVII_LU_OBF; 1.
DR HAMAP; MF_00378; Exonuc_7_L; 1.
DR InterPro; IPR003753; Exonuc_VII_L.
DR InterPro; IPR020579; Exonuc_VII_lsu_C.
DR InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR NCBIfam; TIGR00237; xseA; 1.
DR PANTHER; PTHR30008; EXODEOXYRIBONUCLEASE 7 LARGE SUBUNIT; 1.
DR PANTHER; PTHR30008:SF0; EXODEOXYRIBONUCLEASE 7 LARGE SUBUNIT; 1.
DR Pfam; PF02601; Exonuc_VII_L; 1.
DR Pfam; PF13742; tRNA_anti_2; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00378};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|HAMAP-
KW Rule:MF_00378};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00378};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_00378};
KW Reference proteome {ECO:0000313|Proteomes:UP000194457}.
FT DOMAIN 10..103
FT /note="OB-fold nucleic acid binding"
FT /evidence="ECO:0000259|Pfam:PF13742"
FT DOMAIN 129..435
FT /note="Exonuclease VII large subunit C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02601"
FT COILED 265..325
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 443 AA; 49399 MW; 64652BFBFDBB7EB0 CRC64;
MTPSSSHDAL SVFELNRRAR TSLENTLGEV WVEGELSRVS RPASGHLYFT LKDDRAQVSC
ALFRNRARFV AAPMREGDSV RIRAKVSIFE PRGDYQLIVE TVQEAGRGAL LAALERLKTK
LEAEGIFDNA RELPCPPQHL AIITSASGAA LQDVLAVLQA RWPLLEVSVL PVPVQGDQAA
PAMIRALAAA GRDGRFDALL ITRGGGSLED LWCFNDERLA RAIQASPIPV MSAVGHEIDT
TLSDLAADVR APTPSAAAER LVPDRRDILE RLTTLTRRLE NAQQRQLQQY HQQLDYTASR
LRHPSERLRA QRQRLEDLET RLTRAMHYRL EQAHQRLASD QSRLQQASPE RALRDRREQL
DALTQRLVAA PARQIHAQRQ RLEGIMRELN AVSPLAVLGR GFAILESSSG VVRRSGDTTP
GEVLTARLGE GRLKVEVKRR LKR
//
