ID A0A242A9G6_9ENTE Unreviewed; 468 AA.
AC A0A242A9G6;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Glycosyl hydrolase, family 43 {ECO:0008006|Google:ProtNLM};
GN ORFNames=A5886_002669 {ECO:0000313|EMBL:OTN77569.1};
OS Enterococcus sp. 8G7_MSG3316.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1834191 {ECO:0000313|EMBL:OTN77569.1, ECO:0000313|Proteomes:UP000195043};
RN [1] {ECO:0000313|EMBL:OTN77569.1, ECO:0000313|Proteomes:UP000195043}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=8G7_MSG3316 {ECO:0000313|EMBL:OTN77569.1,
RC ECO:0000313|Proteomes:UP000195043};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genomic Center for Infectious Diseases;
RA Earl A., Manson A., Schwartman J., Gilmore M., Abouelleil A., Cao P.,
RA Chapman S., Cusick C., Shea T., Young S., Neafsey D., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Enterococcus sp. 8G7_MSG3316.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OTN77569.1}.
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DR EMBL; NGKU01000001; OTN77569.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A242A9G6; -.
DR STRING; 1834191.A5886_002669; -.
DR OrthoDB; 177947at2; -.
DR Proteomes; UP000195043; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18820; GH43_LbAraf43-like; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..468
FT /note="Glycosyl hydrolase, family 43"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039376078"
FT ACT_SITE 44
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 212
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 150
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 468 AA; 52704 MW; 52F85DE3A2447C21 CRC64;
MKKRGFLLGF IVATTMSVSG CGSGATEHTV VDDFTPIIDN DGADPWVLQT DDFYYYTKTT
GNNLTLWRSK QLTDVAVGEE KVIWEMPAGL ENIWAPELHY IEGQWIVYFA ANKPSETHRM
YALVNNQADP YSEEWVLEEI AGMDDKFAID GTVLTLGETN YFIWSGWAGY ENVAQDIYIA
EMTSPTSVRG EKQLLSAPEY DWEMKQTPLI NEAPQVIIKE QTVNLVYSAS GSWDNDYALG
LLTMALEDDP LVKDNWTKET EPIFQQTDTV FGPGHNGFAK SKDGSEDWLI YHAARWNHSG
WQRSIRLQAF DWENDGKIII DQPLDEWETQ TLPTGEAKRL RLAAVDAEKK GDLTMVDDET
ALSKQAVSGF ESTNDQLIFK AQIDKAGAYT VIAYVKAPDY LSLSAPIQFN ISNMDSVHTT
QMHPSQYYQP VQVRLSLAEG VEEIILASEI GIDTVQVDRL EIIQLDEE
//
