ID A0A243WCW6_9BACT Unreviewed; 1105 AA.
AC A0A243WCW6;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN ORFNames=BXP70_13945 {ECO:0000313|EMBL:OUJ73501.1};
OS Hymenobacter crusticola.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Hymenobacteraceae;
OC Hymenobacter.
OX NCBI_TaxID=1770526 {ECO:0000313|EMBL:OUJ73501.1, ECO:0000313|Proteomes:UP000194873};
RN [1] {ECO:0000313|EMBL:OUJ73501.1, ECO:0000313|Proteomes:UP000194873}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MIMBbqt21 {ECO:0000313|EMBL:OUJ73501.1};
RA Liang Y., Feng F.;
RT "A new Hymenobacter.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU361154};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUJ73501.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MTSE01000006; OUJ73501.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A243WCW6; -.
DR Proteomes; UP000194873; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154};
KW Reference proteome {ECO:0000313|Proteomes:UP000194873};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..1105
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012331515"
FT DOMAIN 806..1076
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
SQ SEQUENCE 1105 AA; 124368 MW; 9342DCD5FCC2392E CRC64;
MRRPIWTALL LLLASVAASA QEASVYYFPG VSNPPKIYNG EGWDNPLVSE LNRDPARATA
YSFTNEKDAL AGDRAKSRLL SLNGNWDFNF VSKPADAPQD FYKSRVQGWK QLEVPSNWEM
KGYDLPIYKS AKYPFRPVNP PFVPQDYNGV GSYQRTFTVP ANWKDLNVTL HFGGVSSAFK
VWVNGRFLGY GEDSCLPSEF NVTPYLQAGE NTVSVQVIRW SDGSYLEDQD HWRLSGIYRE
VLLLGEPKMR LADFHWQAKL DKQYRDAVLS IRPRLENLTG NDDLKGYLLK AQLYNKNGQP
VFAKPLERTG DAIINEKYPR LDNRKFGLLE TTVANPAKWS DEQPNLYTLV LTLEKDGNVL
EAKSCKLGFR AIEFSKTDGK LLINGKVTYL YGVNRHDHHP TKGMAVSRED IRQDVRTLKQ
FNFNCIRTSH YPNDPYFYDL CDEYGILVMD EANLETHGLG SKLSNDPSWT QAYVSRSTRM
ALRDKNHPSV VFWSLGNESG RGPNHAAMAA WLHDFDITRP VHYEPAQGDP HLDGYIDPSD
PNYPKNHAKR IQVPRDQAYV DMVSRMYPTL TTGALLANQQ NGDNRPIFFC EYSHSMGNAT
GNMKEFWDGW RATKRVIGGC IWEFKDQGLL KRDSLGTPFY AYGGDFQEKY YDDFTIKGIV
ASDGKPHAAL YECKRVYQPA ECVLTDAAKA LIKVTNRHAT ESLNSYNVSL VVREDGKVVA
IKALPHLRLA AGKDTTISLK SYLPKLKAGS EYLADIHFTL AQPTLWADMG HEVASNQFEL
TSLAKPTSKP QQYVALTTRE DASAYTLSGK GFQATFDKTS GALTSYRWNN QEQILAPLLP
HFTRPLTDND RRGWKADKKS KEWFEPSLKL KTITLDQAQK GVATIKSDYT LIDGKAAVQV
VYTLNGAGVL KVDYKLSPQA SLPNMIKVGM QCGIANDYRQ ISWYGRGPWE NYIDRSYAAD
AGIYSQGLQD FMESYVVPQE NSNRTDVRWM LLSSAQKGGL LVVADSLLSM SAWPYTEANI
AKARHTNKLQ EAGFLTLNID LKQIGVGGNT SWDDDAQPVE QYRMPAKPYA YSFYLLPTNS
KKEAASALAK SIKFQSKFSS SVGVK
//
