ID A0A246JG81_9SPHN Unreviewed; 865 AA.
AC A0A246JG81;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Nitrate reductase {ECO:0000313|EMBL:OWQ91656.1};
GN ORFNames=CDQ91_19095 {ECO:0000313|EMBL:OWQ91656.1};
OS Sphingopyxis witflariensis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingopyxis.
OX NCBI_TaxID=173675 {ECO:0000313|EMBL:OWQ91656.1, ECO:0000313|Proteomes:UP000197097};
RN [1] {ECO:0000313|EMBL:OWQ91656.1, ECO:0000313|Proteomes:UP000197097}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14551 {ECO:0000313|EMBL:OWQ91656.1,
RC ECO:0000313|Proteomes:UP000197097};
RX PubMed=12508864; DOI=10.1099/ijs.0.02217-0;
RA Kampfer P., Witzenberger R., Denner E.B., Busse H.J., Neef A.;
RT "Sphingopyxis witflariensis sp. nov., isolated from activated sludge.";
RL Int. J. Syst. Evol. Microbiol. 52:2029-2034(2002).
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OWQ91656.1}.
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DR EMBL; NISJ01000015; OWQ91656.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A246JG81; -.
DR OrthoDB; 9816402at2; -.
DR Proteomes; UP000197097; Unassembled WGS sequence.
DR GO; GO:1990204; C:oxidoreductase complex; IEA:UniProt.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000197097}.
FT DOMAIN 2..58
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 865 AA; 93100 MW; F85A6055CC5C410C CRC64;
MAEAVRTTCA YCGVGCGISA TVTAEREVRI EGDPDHPANR GKLCSKGTHL GETVGLEGRL
LHPMIGKKRA SWDKALDLVA KRFKETIARH GPGSVAFYVS GQLLTEDYYV ANKLMKGFIG
TANIDTNSRL CMSSAVAGHM RAFGEDIVPA TYDDLEAADL IVLVGSNTAW CHPIVYQRIR
ARCEAGAKLV VIDPRRTETA EEADLHLAIR PGSDVALMNG LLQHCREAGI VDEAYLAAHV
AVPDDFWNEL GGGNDLWSVA RTCDVSATDL RRFFDLFAAH PRTVTMFSQG INQATAGTDQ
VNAILNLHLA TGRIGKPGAA PFSITGQPNA MGGREVGGLA TTLAAHMDFA PENRARVQRF
WAAPTIAEKP GLKAVDMFRA IGEGRIKAVW VMATNPAVSM PDANRVRAAL ADCPFVVVSD
VIEHTDTGAF AHVRLPAAAW GEKDGTVTNS DRMVSRQRAL FPLPGEAMPD WWIIKEVARR
MGWKTAFSYD RPADIWREHC RLSIYENEGD RLFALPGAAQ GGNTAYDDMA PFRWGGAHPF
ADGRFSTEDG RARLVAVVQK DLPEPLAKWP LTLNTGRYRD QWHSMTRTGF APKLARHREE
PLVEVHPDDG ARLGLSDGGL ARVDTPQGDS IFRVAFHAGQ RRGEAFVPIH WTDRTSSGGR
TGLLSRPLVD PVSGQPGFKR TPASIAALAT KWRGFLLLAH ELDTHPKCLW ATRVAVPSGA
LWELAGNGDI TSVEALLPRG ERIEAQDAAR GTRRIAVVAG GRLAAALFVT ESGELPPRDW
LIAQLSAPEI APTLLAGRAP GAQVDRGTII CVCFDIGLKT IAAAIREQAL ADVTAIGAAI
GAGTNCGSCR PALARILAEE TSDAA
//
