ID A0A249L5L0_9ACTN Unreviewed; 651 AA.
AC A0A249L5L0;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=B1sIIB91_05885 {ECO:0000313|EMBL:ASY24390.1};
OS Candidatus Nanopelagicus abundans.
OC Bacteria; Actinomycetota; Actinomycetes; Candidatus Nanopelagicales;
OC Candidatus Nanopelagicaceae; Nanopelagicus.
OX NCBI_TaxID=1884916 {ECO:0000313|EMBL:ASY24390.1, ECO:0000313|Proteomes:UP000217210};
RN [1] {ECO:0000313|EMBL:ASY24390.1, ECO:0000313|Proteomes:UP000217210}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MMS-IIB-91 {ECO:0000313|EMBL:ASY24390.1};
RA Neuenschwander S.M., Salcher M., Ghai R., Pernthaler J.;
RT "High microdiversification within the ubiquitous acI lineage of
RT Actinobacteria.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
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DR EMBL; CP016779; ASY24390.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A249L5L0; -.
DR KEGG; nab:B1sIIB91_05885; -.
DR OrthoDB; 7911552at2; -.
DR Proteomes; UP000217210; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF34; PENICILLIN-BINDING PROTEIN 1A; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000217210};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 55..231
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 323..570
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
SQ SEQUENCE 651 AA; 69958 MW; 016D70E1233EDE73 CRC64;
MNNLLAKLPR LGVLITGFSF VAGVFLFGFA YFTVSIPDPN AYVNSQSTII QYADGSEIGR
IGSENRTVVT LAQIPVQVRN AVMAAEDRGF YSNRAISPTA IIRAVWNNVR GGSLQGGSTI
TQQYAKTAFL TPERSITRKV KELVIAIKLE NQFTKDQIFE NYLNTIYFGR GSYGVQTASQ
VYFGTTVDKL TTAQSAVLAS ILRSPGLYDP GFKKENLPRL EKRFTYVLDG MVAQGWLEKE
KAEKIKFPVI NPRVTSGALA GPKGYVISWV ERELNALGFS DEQLMVGGYI IKTTLVKEAQ
EAAVFAVSKE APVKVPENLH IGLVAIKPGT GEILAMYGGQ DYLKYQFNAA TQGITSGGSS
FKAFSLVAAL EQGIPLSSIW NGDSPKVYDD GIGRPYPVNN YGGESWGNLD LLTATEKSVN
TIYVPLGMKA GLEKVVDVAR RAGIPESVEM VASPSVSLGA ASPHVIDLAN AYATFAAQGV
YAKPFIINEV QGPNKGILYQ GRIQAQEVFR KDVMADLTYA FSKVTTSGTA AVVGARVGRP
TAGKTGTSND NASAWFNGYT PEVAASVAFY RDDATQSLNG IGGLTSVTGG SFPARIWAEF
VKKYLAKSLI QEFPAPAFIG GTEPVNFINA VPEMDPALIP PSPSPSPKKK K
//
