ID A0A249LI36_9ACTN Unreviewed; 1069 AA.
AC A0A249LI36;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A1sIIB60_00885 {ECO:0000313|EMBL:ASY28576.1};
OS Candidatus Planktophila lacus.
OC Bacteria; Actinomycetota; Actinomycetes; Candidatus Nanopelagicales;
OC Candidatus Nanopelagicaceae; Planktophila.
OX NCBI_TaxID=1884913 {ECO:0000313|EMBL:ASY28576.1, ECO:0000313|Proteomes:UP000217167};
RN [1] {ECO:0000313|EMBL:ASY28576.1, ECO:0000313|Proteomes:UP000217167}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MMS-IIB-60 {ECO:0000313|EMBL:ASY28576.1};
RA Neuenschwander S.M., Salcher M., Ghai R., Pernthaler J.;
RT "High microdiversification within the ubiquitous acI lineage of
RT Actinobacteria.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP016783; ASY28576.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A249LI36; -.
DR KEGG; plan:A1s21148_01000; -.
DR OrthoDB; 4812256at2; -.
DR Proteomes; UP000217167; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 25..349
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 350..667
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 46..53
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1069 AA; 116803 MW; 57EBEB58BB4C2D9F CRC64;
MIEKYSPQDI AAALQKVGAK VYKPTDEQSA IISIASNPLE PAVVIAGAGS GKTETMAARV
IYLVANGFAR PDQILGLTFT RKASGELAIR IRTRLRQLRA AKLIPEDTPL EVAVTTYHSY
AARLLSEHSI RFGIDADIQP MGDAAMWQLA NDIVRNWEDA SYSNESAVGT VVEDLLGLTK
LMLEHQVSPE AIAAADNEVL EQLAQMSGAT NPEVRKVAKV LSQRTALLPM VERFIQRRQE
SGQLSFDDQM SLAADISVKF SDIGELERAK YSVVLLDEYQ DTSQSQVRML SSLFGGGHPV
MAVGDPCQAI YTWRGASAGT INSFGKYFPK AAGHTGAERY SLLTTFRNDK TILELANVVS
EKIRRESGAD VPPLTPRPGA GDGEIVCGVF ENLDLEATAI AEYFTSLWFD KGRLAKPAEK
RSTFAVLVRK RSQIATIEGA LRAADIPVEV LGLGGLIHVP EVADIVSMLR VIADPDSGAA
LMRHLVGPRI NLGAKDLAAL GAYSRNRTKG SRAESKSLIK KIAAGNPEQA EADDQFLGSL
IDTLDEIGGA DKSTFTEIGY VRLVNFASDL RRLRSRAGGT ITDLINEIES YLSLETEVML
RDGTGSGRRH IDRFLDEAGK FARSGGTLNS FLQWLDIAGK EEGGLKSAAP EVRSDVVQIL
TVHMSKGAEW DVVAVPGLAE GTFPGLNKND PDNWITNERH IPFHLRGDHL ELPRFSFNGA
TKNSDAGKEI TKYADICKAT KTREEIRLGY VAFTRARTHL IATTSWYRDG KDWVEPSTIF
EQVALVAQEH GKIISEVPRP DKDAKNPALE NPLTGIWPRD PLGDTRSAFT AKVELVKNAA
PLDLATAQSV DIETSSWIED ARALIQERAL GVNGVLEIPM PPRLSTSTLV ALHKDPEELA
LTIRRPMPRP QDQYSRRGTA FHLWIEHHFN AATLFDDEDL DFLDPLEPDQ TLESLKQTWL
ASEWGNRSPV AVEVPFEAVL GGVLIRGRID AVYEINGRFE VIDWKTGATT LGESAAVQLA
VYRLAWAKLK GIDPALVSAA FHYVPISKTD RPADLLSEAQ LIALITGAS
//
