ID A0A250KSM5_9GAMM Unreviewed; 722 AA.
AC A0A250KSM5;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Phospholipase D {ECO:0000313|EMBL:BBA34572.1};
GN ORFNames=sS8_2624 {ECO:0000313|EMBL:BBA34572.1};
OS Methylocaldum marinum.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylocaldum.
OX NCBI_TaxID=1432792 {ECO:0000313|EMBL:BBA34572.1, ECO:0000313|Proteomes:UP000266313};
RN [1] {ECO:0000313|EMBL:BBA34572.1, ECO:0000313|Proteomes:UP000266313}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S8 {ECO:0000313|EMBL:BBA34572.1,
RC ECO:0000313|Proteomes:UP000266313};
RA Takeuchi M., Kamagata Y., Hiraoka S., Oshima K., Hattori M., Iwasaki W.;
RT "Genome sequencing of Methylocaldum marinum.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:14445,
CC ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:58608; EC=3.1.4.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000798};
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DR EMBL; AP017928; BBA34572.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A250KSM5; -.
DR KEGG; mmai:sS8_2624; -.
DR OrthoDB; 9814092at2; -.
DR Proteomes; UP000266313; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006793; P:phosphorus metabolic process; IEA:UniProt.
DR CDD; cd09140; PLDc_vPLD1_2_like_bac_1; 1.
DR CDD; cd09143; PLDc_vPLD1_2_like_bac_2; 1.
DR Gene3D; 3.30.870.10; Endonuclease Chain A; 2.
DR InterPro; IPR025202; PLD-like_dom.
DR InterPro; IPR001736; PLipase_D/transphosphatidylase.
DR InterPro; IPR015679; PLipase_D_fam.
DR InterPro; IPR032816; VTT_dom.
DR PANTHER; PTHR18896:SF76; PHOSPHOLIPASE; 1.
DR PANTHER; PTHR18896; PHOSPHOLIPASE D; 1.
DR Pfam; PF00614; PLDc; 1.
DR Pfam; PF13091; PLDc_2; 1.
DR Pfam; PF09335; SNARE_assoc; 1.
DR SMART; SM00155; PLDc; 2.
DR SUPFAM; SSF56024; Phospholipase D/nuclease; 2.
DR PROSITE; PS50035; PLD; 2.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000266313};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 495..514
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 541..567
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 574..595
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 651..670
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 682..703
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 137..164
FT /note="PLD phosphodiesterase"
FT /evidence="ECO:0000259|PROSITE:PS50035"
FT DOMAIN 359..381
FT /note="PLD phosphodiesterase"
FT /evidence="ECO:0000259|PROSITE:PS50035"
SQ SEQUENCE 722 AA; 79448 MW; A2AF0066AFFB60BB CRC64;
MRTFDAHENV ENRILRPGSN CWRSAHASRV SFLIDSSAYF PALREAAIRA RHAIFIVGWD
IDSRTKLVQD GVNDGFPIQL GDFLKSLADH RRGLEVYILN WDFAMLYAAG REVLPIYTLG
WQSHRHLHFY LDGRHPVGAS HHQKIVVIDD AVAFVGGMDL AQERWDTPDH EPNAPYRRRS
NGQAYPPVHD VQMMADGAAA AALGVLVRER WRLATGCELG IGNHAATDPW PPSIAPDIRN
VDIAISRTIP RFNGAGEILE LRQLYLDAIK SAKDTIYIEN QYLTASSIGS LLEFRLRQPS
PPEVVVVSRL TGGGWLEQTT MEVLRARLLR RLRAADRYRK FRIYYPHCEG LDEQCINVHS
KLMVVDDRLA LVGSANLNNR SMGLDTECNL AVEATDAETA TAIAGLRNRL LAEHLGVGTD
RVEQAIRNEG SLIRGIESLR GNAHTLRHLE GEVSPEADAL LPQASVVDPE APIDPDRLID
ELIPVEHRPS ARKRITATLS LLAAIGALAA AWRWGPLHDW LDKETLISVG RTFQRIPATP
VWVIGAYIIA ALTAIPITLV IFVTAVVFGP TSGFVYAILG SLISASLTFA LGQVFGRDLV
RRLGGSRLNT LSRWLRHRGL LAMLTVRLIP VAPFTVVNLV AGASHIRFRD FLLGTIFGMV
PGTLAITVFS DRLAAAVHHP SPVNIALLVG SAIVIGTAAF ATYRWLRRRE VENAACAGGS
VG
//
