ID A0A250KZ42_9GAMM Unreviewed; 694 AA.
AC A0A250KZ42;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=ATP-dependent DNA helicase RecG {ECO:0000256|ARBA:ARBA00017846, ECO:0000256|RuleBase:RU363016};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU363016};
GN Name=recG {ECO:0000256|RuleBase:RU363016};
GN ORFNames=sS8_4939 {ECO:0000313|EMBL:BBA36862.1};
OS Methylocaldum marinum.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylocaldum.
OX NCBI_TaxID=1432792 {ECO:0000313|EMBL:BBA36862.1, ECO:0000313|Proteomes:UP000266313};
RN [1] {ECO:0000313|EMBL:BBA36862.1, ECO:0000313|Proteomes:UP000266313}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S8 {ECO:0000313|EMBL:BBA36862.1,
RC ECO:0000313|Proteomes:UP000266313};
RA Takeuchi M., Kamagata Y., Hiraoka S., Oshima K., Hattori M., Iwasaki W.;
RT "Genome sequencing of Methylocaldum marinum.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA). {ECO:0000256|RuleBase:RU363016}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363016};
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000256|ARBA:ARBA00007504, ECO:0000256|RuleBase:RU363016}.
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DR EMBL; AP017928; BBA36862.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A250KZ42; -.
DR KEGG; mmai:sS8_4939; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000266313; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd17992; DEXHc_RecG; 1.
DR CDD; cd04488; RecG_wedge_OBF; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR045562; RecG_dom3_C.
DR InterPro; IPR033454; RecG_wedge.
DR NCBIfam; TIGR00643; recG; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_dom3_C; 1.
DR Pfam; PF17191; RecG_wedge; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363016};
KW DNA damage {ECO:0000256|RuleBase:RU363016};
KW DNA recombination {ECO:0000256|RuleBase:RU363016};
KW DNA repair {ECO:0000256|RuleBase:RU363016};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU363016};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU363016};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363016};
KW Reference proteome {ECO:0000313|Proteomes:UP000266313}.
FT DOMAIN 285..450
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 483..629
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 694 AA; 76656 MW; C48C8BC4B3261004 CRC64;
MASHNQSLDE LPAATLKGAG AQTLKRLEKL GITTVGDLLF HLPLRYEDRT HVTPIGHLKP
GDHALLEGRI ELAEILPKGR RSLIVRIGDG TGFLDLRFFH YTAKQREQLA RGKRLGCYGE
VRSGYAGLEM THPDYKVIDD AETEVSEQAL TPVYPLTEGV HQKAIRRLTA QALSLADATR
LPELLPDTLI PPASRLQLLD ALRLLHQPPP GCGEASPRLA AARSRLAFEE LLGHHLSLSR
FRAKMQAHRA PLMHTNREAA NQFLRSLPFS LTAAQCRVIS EIETDLASSR PMMRLVQGDV
GSGKTVVAAH AALTALSSNY QVGIMAPTEL LAEQHFRNFR QWLEPFGVHI AFLSGRLKGE
ARKTELEAIS RGTAGIVIGT HALFQEQVVF HRLGLVIIDE QHRFGVHQRL ALREKGLQDG
LYPHQLIMTA TPIPRTLAML GYADLDLSVI DERPPGRTPV KTTVAPTSRR ADIVARITDW
VSRGRQAYWV CTLIEESEVL QCEAAEKTAV TLAEALPDVR VALIHGRLKP AEKEAVMQAF
KERHFDLLVA TTVIEVGVDV PNASLMIIEN AERLGLAQLH QLRGRVGRGP GEAHCVLLYL
PPLSETAKQR LGILRDSDDG FVIAEKDLEL RGPGELLGTR QTGQIQFRIA DLSRDGHLLD
AVAQAAAVLF RDYPDRAERL IRRWVGHSVQ YAGA
//
