ID   A0A250XBY6_9CHLO        Unreviewed;      1159 AA.
AC   A0A250XBY6;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=NFACT RNA-binding domain-containing protein {ECO:0008006|Google:ProtNLM};
GN   ORFNames=CEUSTIGMA_g8028.t1 {ECO:0000313|EMBL:GAX80591.1};
OS   Chlamydomonas eustigma.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=1157962 {ECO:0000313|EMBL:GAX80591.1, ECO:0000313|Proteomes:UP000232323};
RN   [1] {ECO:0000313|EMBL:GAX80591.1, ECO:0000313|Proteomes:UP000232323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIES-2499 {ECO:0000313|EMBL:GAX80591.1,
RC   ECO:0000313|Proteomes:UP000232323};
RA   Hirooka S., Hirose Y., Kanesaki Y., Higuchi S., Fujiwara T., Onuma R.,
RA   Era A., Ohbayashi R., Uzuka A., Nozaki H., Yoshikawa H., Miyagishima S.Y.;
RT   "Acidophilic green algal genome provides insights into adaptation to an
RT   acidic environment.";
RL   Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the NEMF family.
CC       {ECO:0000256|ARBA:ARBA00008318}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GAX80591.1}.
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DR   EMBL; BEGY01000054; GAX80591.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A250XBY6; -.
DR   STRING; 1157962.A0A250XBY6; -.
DR   OrthoDB; 129892at2759; -.
DR   Proteomes; UP000232323; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProt.
DR   Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR   InterPro; IPR021846; NFACT-C.
DR   InterPro; IPR008532; NFACT_RNA-bd.
DR   PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR   PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR   Pfam; PF11923; NFACT-C; 1.
DR   Pfam; PF05670; NFACT-R_1; 1.
DR   Pfam; PF05833; NFACT_N; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|ARBA:ARBA00023054};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Reference proteome {ECO:0000313|Proteomes:UP000232323}.
FT   DOMAIN          533..644
FT                   /note="NFACT RNA-binding"
FT                   /evidence="ECO:0000259|Pfam:PF05670"
FT   DOMAIN          1038..1132
FT                   /note="NFACT protein C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF11923"
FT   REGION          254..295
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          721..741
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          774..1011
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        726..741
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        810..826
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        862..878
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        887..908
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        946..961
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        965..1011
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1159 AA;  126569 MW;  3A5B35527E71A120 CRC64;
     MKQRFTSVDC AAEVACLNNR LLGLRLANVY DLNAKTYVLK LAKSGEDGEK VFLLLESGAR
     FHTTELLREK ADVPSNFTLK LRKHIRTRRL EAVRQLGVDR IVDFVFGSGE GTYHLILEMY
     SQGNIVLTDK NFEVLTLLRS HRDDEKGFAV MARHAYPMHA VRLRQQVQRQ ELWDALNAGM
     EQGQPLKGTL ASLLPYGPAI AEHCIIDSGL DPGSKLSSLE MVTQALEALL PALGRLDTWF
     ATLEQGGSPL GFITVAAPGS KKNPQQSKKK EKQQQNQPGS TCDAAGVPAP PGGESEVPEI
     YQDFNPLRLG QSAGHKVVEF PTFDAALDDF YSKIEGQRAA IARAEAEKNA MGKLDRIRTD
     QASRVTGLQR EAEEAELRGT LIEYNLEAVD AVLLAVNEAL ASGMDWQELD RMIRDERKAG
     NPVACLIHSL QLDQNKVTLI LSNYLDTQED EFDEEANTRP ASKVQVDLSL SAYSNVSVHY
     DSRKKHVVKQ VKTLAANEAA LKAAEKKAQQ QLQQVRNQPS GPVAVRKPLW FEKFNWFISS
     ENYLVISGRD AQQNELIVKR YFRRGDVYVH ADLHGASSTI IKNPDQGKPV PPMTLQQAGC
     ACVCRSRAWD SKIVTSAWWV QHHQVSKAAP TGEYLPTGSF MIRGRKNFLP PQPLVFGFGF
     MFKLEESSIA AHLGERAPRL LLEEEGSQVL DDEGDIEIEE EQDEEEVSAL DRFMEGGIDP
     LARSASSTAG GLQATSSGLG SRTAAASAAN KAQLYKRYGL SEEDAEVSIV GGDVEGGAEA
     GEGGANGQQQ SGRRHMSAKE RELMKKGGGQ FPKQQQQVTA SSKGGENQGK KLDEGVKGKS
     PLVLSATAAA AAARGKKGKQ QKIKERYADQ DDEDRELALA VLASQGDKKT RADRRKERKE
     RKELKRQDGQ TSVPGLNPSA REEAIAKATQ KQFTGGAAAA AEEEPSSSDT AGSNRSSDAG
     DTVGRTEAGM RKRGEGDYRQ PGDNEDDDVK AQQDAHPCSV DPEEEQGELH ATDDAEKAEI
     AALLAEEKTA PELAEEERDR LTVLDALTGV PRAEEGDLLL TALPVCGPYQ ALQGFKYKVK
     LTPGTVKKGK ASKQALELLI RGQDVAPREK ELLRAIPEMD MINAMVGTVK ISMPGMQKLK
     AVEKTNRKIK AKANVAAGI
//