ID A0A250XC24_9CHLO Unreviewed; 470 AA.
AC A0A250XC24;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=protein-S-isoprenylcysteine alpha-carbonyl methylesterase {ECO:0000256|ARBA:ARBA00038928};
DE EC=3.1.1.n2 {ECO:0000256|ARBA:ARBA00038928};
GN ORFNames=CEUSTIGMA_g8041.t1 {ECO:0000313|EMBL:GAX80606.1};
OS Chlamydomonas eustigma.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=1157962 {ECO:0000313|EMBL:GAX80606.1, ECO:0000313|Proteomes:UP000232323};
RN [1] {ECO:0000313|EMBL:GAX80606.1, ECO:0000313|Proteomes:UP000232323}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2499 {ECO:0000313|EMBL:GAX80606.1,
RC ECO:0000313|Proteomes:UP000232323};
RA Hirooka S., Hirose Y., Kanesaki Y., Higuchi S., Fujiwara T., Onuma R.,
RA Era A., Ohbayashi R., Uzuka A., Nozaki H., Yoshikawa H., Miyagishima S.Y.;
RT "Acidophilic green algal genome provides insights into adaptation to an
RT acidic environment.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl
CC ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine +
CC H(+) + methanol; Xref=Rhea:RHEA:48520, Rhea:RHEA-COMP:12125,
CC Rhea:RHEA-COMP:12126, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17790, ChEBI:CHEBI:90510, ChEBI:CHEBI:90511; EC=3.1.1.n2;
CC Evidence={ECO:0000256|ARBA:ARBA00035789};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004586}. Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004653}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004653}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Isoprenylcysteine
CC methylesterase family. {ECO:0000256|ARBA:ARBA00038028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAX80606.1}.
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DR EMBL; BEGY01000054; GAX80606.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A250XC24; -.
DR STRING; 1157962.A0A250XC24; -.
DR OrthoDB; 1651930at2759; -.
DR Proteomes; UP000232323; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR049492; BD-FAE-like_dom.
DR PANTHER; PTHR48081; AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C; 1.
DR PANTHER; PTHR48081:SF36; ALPHA BETA-HYDROLASES SUPERFAMILY PROTEIN; 1.
DR Pfam; PF20434; BD-FAE; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000232323};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 124..335
FT /note="BD-FAE-like"
FT /evidence="ECO:0000259|Pfam:PF20434"
SQ SEQUENCE 470 AA; 51447 MW; D0B05AC8606A90B2 CRC64;
MEGYAMSFCE AAGMRHLNQI IEHMPLMMSV LDPGLKAVKD GEEVFLISHL SLWGASKQAW
TLTKLAFQLL SYLGVGWKWS LAVFQMVIYV TLLLPGFIQM VFFYIFSPRL TKHILYGSGP
RQQLDLYMPP NKSAAHPVVI FITGGAWTIG YKAWGALLAR RLSDQGVIVV CLDYRNFPQG
TALQMLQDVN HGIAWVCRNI GDHCGDPDRV FIVGHSAGAH LGSLALFRQA ERAMLGDQVL
GAEPGWSPTS VRGFIGISGA YDLSKLSEHL HRRGLYRNLF KQIMSIDGQT AYSQLSPLDV
VKSWGRALLP HITLIHGMSD KSAPHECSQE LYDQLREDGV PCALRLIRNK GHTSFLIEDP
MRGGRDILCD MLLQVIGAGH LDGCSGGSFC NSRLVGRPTT RSGGPGEIGS CDNFVPSTAS
SFAMLPIGAM EGLTSRASSV HISVSAQSEY RYPTLCPAIL CSVASRVCPF
//
