ID   A0A250XGB3_9CHLO        Unreviewed;       842 AA.
AC   A0A250XGB3;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE            EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN   ORFNames=CEUSTIGMA_g9258.t1 {ECO:0000313|EMBL:GAX81830.1};
OS   Chlamydomonas eustigma.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=1157962 {ECO:0000313|EMBL:GAX81830.1, ECO:0000313|Proteomes:UP000232323};
RN   [1] {ECO:0000313|EMBL:GAX81830.1, ECO:0000313|Proteomes:UP000232323}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIES-2499 {ECO:0000313|EMBL:GAX81830.1,
RC   ECO:0000313|Proteomes:UP000232323};
RA   Hirooka S., Hirose Y., Kanesaki Y., Higuchi S., Fujiwara T., Onuma R.,
RA   Era A., Ohbayashi R., Uzuka A., Nozaki H., Yoshikawa H., Miyagishima S.Y.;
RT   "Acidophilic green algal genome provides insights into adaptation to an
RT   acidic environment.";
RL   Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC         mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC         Evidence={ECO:0000256|ARBA:ARBA00001678};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000256|ARBA:ARBA00005641}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GAX81830.1}.
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DR   EMBL; BEGY01000071; GAX81830.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A250XGB3; -.
DR   STRING; 1157962.A0A250XGB3; -.
DR   OrthoDB; 313532at2759; -.
DR   Proteomes; UP000232323; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045053; MAN-like.
DR   PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR   PANTHER; PTHR31451:SF60; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00023295};
KW   Reference proteome {ECO:0000313|Proteomes:UP000232323}.
FT   DOMAIN          141..425
FT                   /note="Glycoside hydrolase family 5"
FT                   /evidence="ECO:0000259|Pfam:PF00150"
FT   REGION          565..595
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          700..748
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        700..721
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        732..748
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   842 AA;  92770 MW;  DCC427E2ADF0A8B4 CRC64;
     MIAVSDMAFS QGLKNRLNLS VGVVMKHGRI RRIAGVQLGP MSPACVLIML HILMTQHSTC
     SATQSSTITI EGIEVWTVPP DTAQGVPEEL RSEGFVKRRG HQLVIMAALQ SSSSSNSKAQ
     SSLIPFYYLG FNAYWLIDKA SWGQSWGRQQ VVELFAAAKR LGLRVGRTWA FNSKLPSEPG
     VYDEGQLEAL DFIIYAAGRY HVRLILALGN FWPAYFGPER WLLYGKGYTA GQDILDFYGD
     LPTRELYKAH ISFMVNRVNK FTGLKYKDDP TIMGWDVMNE PRCPGCLDDA SHDTVASWMC
     EMTHHLRSQD GNHLILLGSE GFFMADNSSS HLNQFNPGAG AQCEGEDWPS IGSIPDLDVS
     TLHVYERHME LQPVPPVGRS YLRDPDWIYC DFECYINWMV RFIQLNLEVN EREMRKPVII
     EEFGLTWWRN LASDLRVVVE VVRQLLVHSS DTGGALAGAM IWSAALNDSD DQDGYNVKID
     GWPSSLGDAS ASTPVVHYPI SPPVLGSYKP FIVPFYDQPG AGSQFLAVQP DGPHRYYCRS
     NWSNNTTAAA ATAIAVNSSY VEHSDNSDTV LSSQPATRSD VKAVSPSSHP SPPSATAIMN
     SDSALISMKQ SFANRSEWTA MNSTLLLTAS NTSDLEMEPL LSPSNSIGQL NHNDVSGSSP
     AALIANDAHE GGGTTSGGGY HPVVVKKVVI TLSPVPMMIA GDTSQPTTRN MTTSQDDLVT
     SPVDMNGDEP QDVVQPETSS PDAAANQHSG VRRQFLSHHM DGAIIKHLRS RFSTRRLRWQ
     QDQLDGFRRG WQRGDCANTA SRTWRPIPIN TSVVDVAAFR SFTAPLRLVD MLGNATRQLL
     HG
//