ID A0A250XLE7_9CHLO Unreviewed; 2122 AA.
AC A0A250XLE7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=EF-hand domain-containing protein {ECO:0000259|PROSITE:PS50222};
GN ORFNames=CEUSTIGMA_g11322.t1 {ECO:0000313|EMBL:GAX83898.1};
OS Chlamydomonas eustigma.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=1157962 {ECO:0000313|EMBL:GAX83898.1, ECO:0000313|Proteomes:UP000232323};
RN [1] {ECO:0000313|EMBL:GAX83898.1, ECO:0000313|Proteomes:UP000232323}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2499 {ECO:0000313|EMBL:GAX83898.1,
RC ECO:0000313|Proteomes:UP000232323};
RA Hirooka S., Hirose Y., Kanesaki Y., Higuchi S., Fujiwara T., Onuma R.,
RA Era A., Ohbayashi R., Uzuka A., Nozaki H., Yoshikawa H., Miyagishima S.Y.;
RT "Acidophilic green algal genome provides insights into adaptation to an
RT acidic environment.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141,
CC ECO:0000256|RuleBase:RU003808}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141, ECO:0000256|RuleBase:RU003808}.
CC -!- SIMILARITY: Belongs to the calcium channel alpha-1 subunit (TC
CC 1.A.1.11) family. {ECO:0000256|RuleBase:RU003808}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAX83898.1}.
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DR EMBL; BEGY01000110; GAX83898.1; -; Genomic_DNA.
DR STRING; 1157962.A0A250XLE7; -.
DR OrthoDB; 2966630at2759; -.
DR Proteomes; UP000232323; Unassembled WGS sequence.
DR GO; GO:0005891; C:voltage-gated calcium channel complex; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005245; F:voltage-gated calcium channel activity; IEA:InterPro.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProt.
DR Gene3D; 1.10.287.70; -; 4.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 1.20.120.350; Voltage-gated potassium channels. Chain C; 4.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR002077; VDCCAlpha1.
DR InterPro; IPR027359; Volt_channel_dom_sf.
DR PANTHER; PTHR45628; VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1; 1.
DR PANTHER; PTHR45628:SF7; VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1; 1.
DR Pfam; PF00520; Ion_trans; 4.
DR PRINTS; PR00167; CACHANNEL.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF81324; Voltage-gated potassium channels; 4.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS50096; IQ; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|PIRSR:PIRSR602077-1};
KW Calcium channel {ECO:0000256|ARBA:ARBA00022673,
KW ECO:0000256|RuleBase:RU003808};
KW Calcium transport {ECO:0000256|ARBA:ARBA00022568,
KW ECO:0000256|RuleBase:RU003808};
KW Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR602077-1};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000232323};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448};
KW Voltage-gated channel {ECO:0000256|ARBA:ARBA00022882,
KW ECO:0000256|RuleBase:RU003808}.
FT TRANSMEM 72..90
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 110..132
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 144..166
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 201..220
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 367..390
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 488..510
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 522..546
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 566..586
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 607..632
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 764..791
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1018..1045
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1057..1077
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1089..1110
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1162..1183
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1249..1270
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1290..1314
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1363..1390
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1402..1423
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1435..1454
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1491..1515
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1622..1648
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1662..1697
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT REGION 800..825
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1836..1907
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 800..814
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1836..1854
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1859..1907
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1263
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR602077-1"
SQ SEQUENCE 2122 AA; 236175 MW; 676B0CC817018A91 CRC64;
MILSSRARPP DQPSNFNFKR ATLPFYLWIR EAATFYPWEV VSSYELAPKA LFFLPKDNFI
RKPLIRLVRW KLFDWTMLLV ILANCVTLAM DSNKPGFGES ALGQSLSKSN YVFIAIFMVE
ATCKIVALGF LLNKYTYLRN PWNVLDFVVV CMGILELTSF GNYTFIRSFR AFRPLRAATK
IESLKLIVEA LVRSLPMLGD VVILAAFYMS IFGIGCLELF RGKFGYRCGT PDFSNAYTKY
STVGDQIIQN VSYSVASQDQ SQLCKDPMAV GHSWINTSLG PQAAPFEYIG GNQYGFVCPW
IPSDNPYSNN YPNGLLCVLY GNPDIGGYRN FDNIFYSFIQ VFQHLACQDW SQIMYSTQAT
MSWWTWLLHY TMVLIGGYLI ANLTLAVVFI NFNKNYSAAK SASSKSEAAA ARAKFEEVRF
EELELLDDQS LGFRSSPQCA YPETYNAQST APLPIDEVLQ PPARQMGVLT RTWERMRDFC
YIIQDSQLFQ HTTTTIIILN AITLAIIWYD MPDVVANATQ DANFAFTVYF FLEMVIKLVG
LGFTAYLSDN FNIFDALVTT MGVVEMIIIL VPNVTITSGM SVFRAFRLLR IFRLARKWKS
LNRILRVLLK SLAAVGWVTV LLILFLFIVG LLGMTFFGYK LDTCGMVPGS VQLCPPGLTW
RDCPSHFDCY IACDESLALQ WYQVPGSPYG NQAYCEVFPR AYGSNYSVLD VKSTVQFWAQ
VGKPTTYMPN YDDIYEAIIS GFIMLTQDNW DVNMKSIMVM TGPWLPALFT IITMIIGIYT
VLNLFLAVLL ANLDEITESA PNSPSHSHAG DTNISDGPKT DRGPEIKVAT KPSAALFQPG
GGSILDKATT VHTSFWVPKD MQKKLTSNLL RNEMELAMVY NTSQFHKMST QPGPVKTSLV
TYSKSSVVPL PPILQLPLRP LEEHDCWDEV HDNALQYSSA PYPLSVNKSS LSFSARSRSF
MCRTPSLCNM VAPPPAPSML LHPVPVAGGE APLEGKAIFF LNRENKLRQL LYAVVNNVYF
EFAMLFIILL SSLELCFATV LVVPGSVEDR LLNALDIFFT ATFTAEALMK VMAYGFLLNG
SHSYLRQPWN VLDFVVVVVG MLILALEHVM SPSDIKWLRA FRAIRVLRPL RVATRIDGIR
VVVTAMARAI PAIGEIFLVG SLFYYIFAVL GVNLMCGLFL GCYSGGSLLD PYYLVEPGAT
INKTWCEVDG GNHNISNSYY HASLNITVPE WSLSAAWGAN GILQRFDNVI MALWVLFQVS
SLENWSPVMI QAMDSTSLNS QPLFNANVWM ALYFLVFVVL GVFFILNMVI GVAINQFNKL
KEESGKSAFL TDSQQQWLTV QRMMAMVKPK AKLKAPSNAF RRFIFRAVVS GTFDSFMIVV
IMANIFSMMM TYEGESQQWQ DALTLANAVF TGVYVLEMFL KWIAVGLPRY FRDGWCQFDL
VVVVVSVAGV IMDYTTSENL SIMPLLRVLR VLRIIKLVPK ARGLKLMTLT LMWSLPALFN
VAAVLFLFMY IYAIIGMNIF GNLKFQSNLD NDANFQTFPS AMFLLFRMQS GENWDYLMTD
CMVQQSCIEI TQHLVHTFPD NGTNITFWPG QYLDSDSNAE LLALVPLTAQ NDRCTPSAWV
TALYFCSYMM VCVYLVLELV IGVIIENIEM LQKIEKMRIT QQHLEMFVEA WEDIDVNGSG
YIHAADLTSV LLQVLPPLGV KGVSHATAKI QQMLQDAEIP LRNNGRVHFI EALHSLSGRV
VGDALPDSEE IAIRNRMVQK LPKDELPPKF HVGDYYAALY VKAAIKGYLV RAHLKPAFEK
YELSVRGGGG PTSSFSTVRA QRMMILKPLS SLLVRSGTEK EHEAQAERSK GGAIGSKEDT
ISIDSSSRGG GSSSSRGQGG YAASCGTSGP NSSLHENNPT PPVFNLEMSP FSFQQQSAEE
VFSGDLKYEN QPTAAMLPSI ATCVDNENQP TTAMLPSIAT CVDNENQPTA AMLPSIATCV
NNENQPTAAM LPSIATCVDN ENQPTAAMLP SIATRVDNEN QPTAAMLPSI ATCVDNENQP
TTAMLPSIAT CVDNEDQTPA TAMSPAITGS VDYKDQPISM LPSIATCVDS EDQKPATAML
PDINGSVDRK DQPVSMLLSI MP
//
