ID A0A251P3X6_PRUPE Unreviewed; 1236 AA.
AC A0A251P3X6;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:ONI06243.1};
GN ORFNames=PRUPE_5G049500 {ECO:0000313|EMBL:ONI06243.1};
OS Prunus persica (Peach) (Amygdalus persica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=3760 {ECO:0000313|EMBL:ONI06243.1, ECO:0000313|Proteomes:UP000006882};
RN [1] {ECO:0000313|EMBL:ONI06243.1, ECO:0000313|Proteomes:UP000006882}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nemared {ECO:0000313|Proteomes:UP000006882};
RX PubMed=23525075; DOI=10.1038/ng.2586;
RA Verde I., Abbott A.G., Scalabrin S., Jung S., Shu S., Marroni F.,
RA Zhebentyayeva T., Dettori M.T., Grimwood J., Cattonaro F., Zuccolo A.,
RA Rossini L., Jenkins J., Vendramin E., Meisel L.A., Decroocq V.,
RA Sosinski B., Prochnik S., Mitros T., Policriti A., Cipriani G., Dondini L.,
RA Ficklin S., Goodstein D.M., Xuan P., Del Fabbro C., Aramini V., Copetti D.,
RA Gonzalez S., Horner D.S., Falchi R., Lucas S., Mica E., Maldonado J.,
RA Lazzari B., Bielenberg D., Pirona R., Miculan M., Barakat A., Testolin R.,
RA Stella A., Tartarini S., Tonutti P., Arus P., Orellana A., Wells C.,
RA Main D., Vizzotto G., Silva H., Salamini F., Schmutz J., Morgante M.,
RA Rokhsar D.S.;
RT "The high-quality draft genome of peach (Prunus persica) identifies unique
RT patterns of genetic diversity, domestication and genome evolution.";
RL Nat. Genet. 45:487-494(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13066;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01341}.
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DR EMBL; CM007655; ONI06243.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A251P3X6; -.
DR EnsemblPlants; ONI06243; ONI06243; PRUPE_5G049500.
DR Gramene; ONI06243; ONI06243; PRUPE_5G049500.
DR Proteomes; UP000006882; Chromosome g5.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:InterPro.
DR CDD; cd21407; 1B_UPF1-like; 1.
DR CDD; cd18039; DEXXQc_UPF1; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR CDD; cd21400; ZBD_UPF1-like; 1.
DR Gene3D; 2.40.30.230; -; 1.
DR Gene3D; 6.10.140.1240; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047187; SF1_C_Upf1.
DR InterPro; IPR040812; UPF1_1B_dom.
DR InterPro; IPR018999; UPF1_CH/ZBD.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR PANTHER; PTHR10887:SF364; REGULATOR OF NONSENSE TRANSCRIPTS 1; 1.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF18141; UPF1_1B_dom; 1.
DR Pfam; PF09416; UPF1_Zn_bind; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51997; UPF1_CH_RICH; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE-
KW ProRule:PRU01341}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000006882};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU01341};
KW Zinc-finger {ECO:0000256|PROSITE-ProRule:PRU01341}.
FT DOMAIN 130..289
FT /note="Upf1"
FT /evidence="ECO:0000259|PROSITE:PS51997"
FT DOMAIN 495..627
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT REGION 1..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..230
FT /note="C4"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
FT REGION 956..1012
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1208..1236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 956..972
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1236 AA; 135562 MW; 6C4B527FAA1871C2 CRC64;
MDSQPNNLFE TASQPDTGND AYTFLEFNTQ GEDFDYPEFR DPIRSPVAWP TPSDSLSDPT
DRDRGGGGVG SDHQSDASPV SAAPGSATKA RAGGSGSSGG NNQVVDVLAG GMSVLNFEDT
GDDDNYEYGK GNFTEHACRY CGVSNPACVV RCNVPSCRKW FCNSRGNTSG SHIVNHLVRA
KHKEVCLHKD SPLGETILEC YNCGCRNVFL LGFISAKTES VVVLLCREPC LSVNALKDMN
WDLSQWCPLI DDRCFLQWLV KVPSEQEQLR ARQISAQQIN KVEELWKTNP DASLEDLEKP
GVDDEPQPVV LKYEDAYQYQ NVFAPLIKLE ADYDKMMKES QSKDNVTIRW DIGLNKKRIA
YFVFPKEDNE LRLVPGDELR LRYSGDAAHP AWQSVGHVIK LTAQEEVALE LRASQGVPVD
VNHGFSVDFV WKSTSFDRMQ GAMKAFAVDE TSVSGYIYHH LLGHEVEVQM VRNTLPRRFG
APGLPELNAS QVFAVKSVLQ KPISLIQGPP GTGKTVTSAA IVYHMAKQGQ GQVLVCAPSN
VAVDQLAEKI SATGLKVVRL CAKSREAVSS PVEHLTLHYQ VRHLDTSEKS ELHKLQQLKD
EQGELSSSDE KKYKALKRAT EREISQSADV ICCTCVGAGD PRLANFRFRQ VLIDESTQAT
EPECLIPLVL GAKQVVLVGD HCQLGPVIMC KKAARAGLAQ SLFERLVLLG VKPIRLQVQY
RMHPALSEFP SNSFYEGTLQ NGVTINERQS SGIDFPWPVP NRPMFFYVQM GQEEISASGT
SYLNRTEAAN VEKIVTTFLR SGVVPSQIGV ITPYEGQRAY IVNYMSRNGA LRQQLYKEIE
VASVDSFQGR EKDYIILSCV RSNEHQGIGF LNDPRRLNVA LTRARYGIVI LGNPKVLSKQ
PLWNSLLTHY KEHECLVEGP LNNLKQSMVQ FPKPKKIYND RRLFFGGGPG VIPNDSYGSI
ASSGQSADRR STRGRGSYLP PGPPNGAHKP GVHPAGYPMP RAPLSPFHGG PLSQPYAIPT
RGAVHGPVGA VPHVPQPGSR GFGAGRGNAG APIGSHLPHQ QGTQQNVGNL GSTFNFPALE
NPNSQPSVGG PLSQPGFVNN MPQGPSQTFR DGFSMAGMSQ EFLGDDFKSQ GSHVPYNVAD
FSTQASQSGY AVDYVTQGAQ GGFPGNFMNQ NSQAGYSRFG TGNDFMSQGF MNSLYSQPFA
HYNTQPMNLQ APQQQQQQQP PQQGQSSQNQ KIHYNG
//
