ID A0A251PEG6_PRUPE Unreviewed; 782 AA.
AC A0A251PEG6;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00012265};
DE EC=2.5.1.114 {ECO:0000256|ARBA:ARBA00012265};
DE AltName: Full=tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00031315};
GN ORFNames=PRUPE_4G021000 {ECO:0000313|EMBL:ONI09966.1};
OS Prunus persica (Peach) (Amygdalus persica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=3760 {ECO:0000313|EMBL:ONI09966.1, ECO:0000313|Proteomes:UP000006882};
RN [1] {ECO:0000313|EMBL:ONI09966.1, ECO:0000313|Proteomes:UP000006882}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nemared {ECO:0000313|Proteomes:UP000006882};
RX PubMed=23525075; DOI=10.1038/ng.2586;
RA Verde I., Abbott A.G., Scalabrin S., Jung S., Shu S., Marroni F.,
RA Zhebentyayeva T., Dettori M.T., Grimwood J., Cattonaro F., Zuccolo A.,
RA Rossini L., Jenkins J., Vendramin E., Meisel L.A., Decroocq V.,
RA Sosinski B., Prochnik S., Mitros T., Policriti A., Cipriani G., Dondini L.,
RA Ficklin S., Goodstein D.M., Xuan P., Del Fabbro C., Aramini V., Copetti D.,
RA Gonzalez S., Horner D.S., Falchi R., Lucas S., Mica E., Maldonado J.,
RA Lazzari B., Bielenberg D., Pirona R., Miculan M., Barakat A., Testolin R.,
RA Stella A., Tartarini S., Tonutti P., Arus P., Orellana A., Wells C.,
RA Main D., Vizzotto G., Silva H., Salamini F., Schmutz J., Morgante M.,
RA Rokhsar D.S.;
RT "The high-quality draft genome of peach (Prunus persica) identifies unique
RT patterns of genetic diversity, domestication and genome evolution.";
RL Nat. Genet. 45:487-494(2013).
RN [2] {ECO:0000313|EMBL:ONI09966.1}
RP NUCLEOTIDE SEQUENCE.
RA Verde I., Jenkins J., Dondini L., Micali S., Pagliarani G., Vendramin E.,
RA Paris R., Aramini V., Gazza L., Rossini L., Bassi D., Troggio M., Shu S.,
RA Grimwood J.H., Tartarini S., Dettori M.T., Schmutz J.;
RT "WGS assembly of Prunus persica.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent transferase that acts as a
CC component of the wybutosine biosynthesis pathway. Wybutosine is a hyper
CC modified guanosine with a tricyclic base found at the 3'-position
CC adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes
CC the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-
CC adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14)
CC to produce wybutosine-86. {ECO:0000256|ARBA:ARBA00037786}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine =
CC 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) +
CC H(+) + S-methyl-5'-thioadenosine; Xref=Rhea:RHEA:36355, Rhea:RHEA-
CC COMP:10164, Rhea:RHEA-COMP:10378, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:64315,
CC ChEBI:CHEBI:73550; EC=2.5.1.114;
CC Evidence={ECO:0000256|ARBA:ARBA00036405};
CC -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004797}.
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DR EMBL; CM007654; ONI09965.1; -; Genomic_DNA.
DR EMBL; CM007654; ONI09966.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A251PEG6; -.
DR EnsemblPlants; ONI09965; ONI09965; PRUPE_4G021000.
DR EnsemblPlants; ONI09966; ONI09966; PRUPE_4G021000.
DR Gramene; ONI09965; ONI09965; PRUPE_4G021000.
DR Gramene; ONI09966; ONI09966; PRUPE_4G021000.
DR UniPathway; UPA00375; -.
DR Proteomes; UP000006882; Chromosome g4.
DR GO; GO:0102522; F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 1.
DR Gene3D; 3.30.300.110; Met-10+ protein-like domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR23245; TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR23245:SF25; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG; 1.
DR Pfam; PF01344; Kelch_1; 1.
DR Pfam; PF13418; Kelch_4; 2.
DR Pfam; PF02475; Met_10; 1.
DR SMART; SM00612; Kelch; 3.
DR SUPFAM; SSF117281; Kelch motif; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000006882};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 517..776
FT /note="SAM-dependent methyltransferase TRM5/TYW2-type"
FT /evidence="ECO:0000259|PROSITE:PS51684"
SQ SEQUENCE 782 AA; 87048 MW; ECAAE15E97676C55 CRC64;
MAISGEPEEN LFLWGHSACA LEAKNQNGVL VFGGFGGIGR HGRRNHSWLV DPFSGTVKAI
NVESSPSPRL GHTSSLVGDC VFVIGGRSDP EKILNDVWVL NTSKKEWKFL ECSGDVFPPR
HRHAAAVVGS KIYVFGGLNN DTITSSLHVL DTDNLQWKEL FVSGEHPCAR HSHSMVACGS
QLYIFGGYNG EQTLGDLYVY NIQTCKWKKE KAAGRSPHAR FSHSMFVYRN YLGVIGGCPV
RQHCQELAIL DLKQSVWRHA KLESTSEDLF VRSTANIVGD DLVMIGGGAS CYAFGTKFSK
PVKINLLPLM SIDNNIKPVV RERDAHRYEM VNSEKSGRFQ DPQAEDAQSL TEALDLNFES
DFPGENGIGH QVESYWILQL ERKYAKVGKD ILKKFGWLDL GRKVYSRKGG LHICFPVNGK
FSGVFKENKR PLTDLSEGES DHFVKPVIGE ECLLNAVTCS KALDILKECG ATKLADEVLE
VRRAAKSPLK VMNEAVGSLI KDKGLPEELL EELPARWEQL GDIVVLPATS FKNPLWDSMR
EELWPVIAKS VNAHRLARQG RVASNGTRDS TLEILLGDNG WVDHRENGIL YSFDATKCMF
SWGNLSEKLR VASLNCRDEI VVDLFAGIGY FVLPFLVRAN AKLVYACEWN PHAVEALRRN
LQANSVSDRC IILEGDNRTV APKGVADRVC LGLIPTSAGS WVTAVRALRS EGGMLHVHGN
VKDSEESLWT KHVSESVGEI AKSEGHCWEV SIEHLERVKW YAPHIRHLVA DVRCRQSQRW
PN
//
