ID A0A251RD54_PRUPE Unreviewed; 1176 AA.
AC A0A251RD54;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
GN ORFNames=PRUPE_1G457500 {ECO:0000313|EMBL:ONI33989.1};
OS Prunus persica (Peach) (Amygdalus persica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=3760 {ECO:0000313|EMBL:ONI33989.1, ECO:0000313|Proteomes:UP000006882};
RN [1] {ECO:0000313|EMBL:ONI33989.1, ECO:0000313|Proteomes:UP000006882}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nemared {ECO:0000313|Proteomes:UP000006882};
RX PubMed=23525075; DOI=10.1038/ng.2586;
RA Verde I., Abbott A.G., Scalabrin S., Jung S., Shu S., Marroni F.,
RA Zhebentyayeva T., Dettori M.T., Grimwood J., Cattonaro F., Zuccolo A.,
RA Rossini L., Jenkins J., Vendramin E., Meisel L.A., Decroocq V.,
RA Sosinski B., Prochnik S., Mitros T., Policriti A., Cipriani G., Dondini L.,
RA Ficklin S., Goodstein D.M., Xuan P., Del Fabbro C., Aramini V., Copetti D.,
RA Gonzalez S., Horner D.S., Falchi R., Lucas S., Mica E., Maldonado J.,
RA Lazzari B., Bielenberg D., Pirona R., Miculan M., Barakat A., Testolin R.,
RA Stella A., Tartarini S., Tonutti P., Arus P., Orellana A., Wells C.,
RA Main D., Vizzotto G., Silva H., Salamini F., Schmutz J., Morgante M.,
RA Rokhsar D.S.;
RT "The high-quality draft genome of peach (Prunus persica) identifies unique
RT patterns of genetic diversity, domestication and genome evolution.";
RL Nat. Genet. 45:487-494(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
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DR EMBL; CM007651; ONI33989.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A251RD54; -.
DR STRING; 3760.A0A251RD54; -.
DR EnsemblPlants; ONI33989; ONI33989; PRUPE_1G457500.
DR Gramene; ONI33989; ONI33989; PRUPE_1G457500.
DR eggNOG; KOG1956; Eukaryota.
DR OrthoDB; 167550at2759; -.
DR Proteomes; UP000006882; Chromosome g1.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03363; TOPRIM_TopoIA_TopoI; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 3.30.65.10; Bacterial Topoisomerase I, domain 1; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR HAMAP; MF_00952; Topoisom_1_prok; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005733; TopoI_bac-type.
DR InterPro; IPR028612; Topoisom_1_IA.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034149; TOPRIM_TopoI.
DR NCBIfam; TIGR01051; topA_bact; 1.
DR PANTHER; PTHR42785:SF1; DNA TOPOISOMERASE; 1.
DR PANTHER; PTHR42785; DNA TOPOISOMERASE, TYPE IA, CORE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13368; Toprim_C_rpt; 2.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000006882};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 363..478
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT REGION 236..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1021..1043
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1153..1176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 261..295
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1161..1176
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1176 AA; 130281 MW; C835453177C98D38 CRC64;
MAMIHFQRTL SLTAPTSSAS SFVCSNTLAS SALRLRRSMA KLQYRAFETY PCVCLPSSPI
LTASKYRNYS QLKLKKSGGS CMIMINPAKV GPSFHSFRRS GDGSERAFSN LKPGSLNSLG
PGNVISPGSF GSVANYSIGG FAKRFFSQVP HVTSKLESVG AQNGKYSVST SFFKQLSKRR
KREKALAAQS KNEATISSIS KDVDVEASTE RQIGSEKGDG KLGSFLSNSL IINDNHVPKV
KGKKRSKSKK TKEQISAANA PAEAAATQSS KGTSQTKKSG STKSGKKSAQ DSELNFTGKV
PVEISDGSSI KPQPKKKSGN SSRKGKSITS DSKSPQKHIP QKMGKVKPQG KMTLKQLYPA
TGRTVVVVES ATKAKVIQGY LGDMFEVLPS YGHVRDLAAR SGSVRPDDDF SMVWEVPSAA
WTHLKSIKVA LTGAENLILA SDPDREGEAI AWHIIEMLQQ QDALREDINV ARVVFHEITE
TSIKSALQAP REIDENLVHA YLARRALDYL IGFNISPLLW RKLPGCQSAG RVQSAALALL
CDREMEIDEF KQQEYWTAEI ELNQKEPGSS VNAPDFSAHL THFDSKKLNQ FSIDSHTKAK
EIEQQINSQN FQVVSSKKNK MRKNPPTPYI TSTLQQDAAN KLHFSAAYTM KLAQKLYEGV
QLSDGKAAGL ITYMRTDGLH ISDEACKDVR SLVIQRYGQN FASESPRKFF KKVKNAQEAH
EAIRPTDVRR LPSLFRGMLD EDSLKLYTLI WCRTVACQME PATIEQIQLD IGNAGESIVF
RSSCSRVEFL GYQAVFEDVE AQAVKYKENE GNSRNEGFGL LNSLKRGDPL YVGKVELKEH
HTQPPARYSE ASLVKKLEEL GIGRPSTYAS TLKVLQDRNY VTVKSRVLHP EFRGRMVSAF
LGHHFSEVTD YSFTADMETE LDNVSAGLTE WKGLLRDYWT RFSAYCDRTS TVHIHQVEKM
MEKKFGDFLF ASLPDQSRTC PCCMEGNLIF KVSRFGAGYF IGCDQHPKCK YIAKTLYGDD
EEEESTPKNS SMEEPKVLGL SPGSNEKVLL KNGPYGSYVQ LGEDRKGNSP KRASVSHIKD
VGSITLEDAL ELMRYPVTLG NHPEDGQPVI IKVARVGFAV RHRRTIASVP KVYKNKKPSE
ITLEDALELL SSSNVRRSGR PKSKPKVEEE VMLEAV
//
