ID A0A251RJ34_PRUPE Unreviewed; 2208 AA.
AC A0A251RJ34;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
GN ORFNames=PRUPE_1G563000 {ECO:0000313|EMBL:ONI35966.1};
OS Prunus persica (Peach) (Amygdalus persica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=3760 {ECO:0000313|EMBL:ONI35966.1, ECO:0000313|Proteomes:UP000006882};
RN [1] {ECO:0000313|EMBL:ONI35966.1, ECO:0000313|Proteomes:UP000006882}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nemared {ECO:0000313|Proteomes:UP000006882};
RX PubMed=23525075; DOI=10.1038/ng.2586;
RA Verde I., Abbott A.G., Scalabrin S., Jung S., Shu S., Marroni F.,
RA Zhebentyayeva T., Dettori M.T., Grimwood J., Cattonaro F., Zuccolo A.,
RA Rossini L., Jenkins J., Vendramin E., Meisel L.A., Decroocq V.,
RA Sosinski B., Prochnik S., Mitros T., Policriti A., Cipriani G., Dondini L.,
RA Ficklin S., Goodstein D.M., Xuan P., Del Fabbro C., Aramini V., Copetti D.,
RA Gonzalez S., Horner D.S., Falchi R., Lucas S., Mica E., Maldonado J.,
RA Lazzari B., Bielenberg D., Pirona R., Miculan M., Barakat A., Testolin R.,
RA Stella A., Tartarini S., Tonutti P., Arus P., Orellana A., Wells C.,
RA Main D., Vizzotto G., Silva H., Salamini F., Schmutz J., Morgante M.,
RA Rokhsar D.S.;
RT "The high-quality draft genome of peach (Prunus persica) identifies unique
RT patterns of genetic diversity, domestication and genome evolution.";
RL Nat. Genet. 45:487-494(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC an acidic residue or by a hydroxy-amino-acid residue, the
CC phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC Evidence={ECO:0000256|ARBA:ARBA00000451};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM007651; ONI35966.1; -; Genomic_DNA.
DR EnsemblPlants; ONI35966; ONI35966; PRUPE_1G563000.
DR Gramene; ONI35966; ONI35966; PRUPE_1G563000.
DR eggNOG; KOG1849; Eukaryota.
DR Proteomes; UP000006882; Chromosome g1.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR InterPro; IPR005314; Peptidase_C50.
DR InterPro; IPR030397; SEPARIN_core_dom.
DR PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR Pfam; PF03568; Peptidase_C50; 1.
DR PROSITE; PS51700; SEPARIN; 1.
PE 4: Predicted;
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000006882}.
FT DOMAIN 1977..2071
FT /note="Peptidase C50"
FT /evidence="ECO:0000259|PROSITE:PS51700"
FT REGION 1710..1729
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2208 AA; 248842 MW; C7665913024CA37B CRC64;
MDETSIISKL ETSCSSGLHP LVSDYLQPFT QLQNPKKTKK STKSQDQQTL LRSLAKKFLP
FINRTLSLLP KRLAGFSKLD DEFTLELFDI YRLCLDCLDS LSSVLSGTPY SFNLQRVRMV
TCLEACGRYK DAESEGFRVL ESLKAIEFGS NTRVKSDRRF VPDVEKGSGD KDLGSLVGGI
VVTLVRCAGM NQSKDSEVYR RVLCLVEEVM PWFRVLDANT YEKLHRMLVS SLSKCTQFLV
GELSSFEGDL VKMFCLVTMT EYAKSSMKDQ IFKFAHRICS SLFLFQKDRC LLIDILFCLL
DSLVRECKVE VENTGKEFVE LIAYCAKKCR TTNTNLCSII GSHLNELAGD FHQVRTPFHL
ILRLYASGLH FFDRSMKSKA GGGAIRILHD DGDAMNRLSD LLGLLRSYFQ IGHNEDTVLS
NLQLISKSAA SMSQLQRDRK DYILSYFNAL KFLCQPLTEL VNSGKKEILT DNEAASVSTE
LCDIQGAFHQ FYDVFVFFQT CTYEVDRDVF DDRDIFDDNS IISVALASFT LSIRTKLNIQ
KSVQILENVI TSDWIQPNGL KHLYVSLYNT GVLFYRNKEL KEASEALKFC CKASWTCVIC
VCDMFVHKVK VPQVDLSEDA IVDFFDECCK RSAFLLDVLN QLQSHDVKRT ILESFENWSI
AANLFQRLPG PLSLVKQWVK MECKHYKNVD VEDDAPTLYS LLSSSKKVTK KTIEIVLEQE
LLAYEEMNDV NPEFCQKMQM KIIDILLQYV HVTPDSCLQK SRILLRKGRA LRLSGISGLK
GCIQCLSDAI SSLNEMYDET YIHEISPCHQ LAVAYCLRAL CTQEAEPNSK QVLEDISSAI
NLWLGISTRN NCSPDDKCSM VSESIMLLLY NAIDLLSIKG CMDFHNDIHR LMIRLFKWRD
VPLEKCVARL WECRRISHGL CASPVNEAFI MNLSDHCGEN SKSIEFWVDC LKESKPLLLA
FQYNLSSVSP NFPRGSCNYE SSFRSDITID EVKEAAFELI SSVPVLSSSA YIAGYLYYDL
CERLVSNGRL IEALSYAKEA YQLRAKLFRE KFMYSSEQSK TCNEAGGSGE KLTYHIQDMH
MHISVASEFW SFDASLCDLE RCYLSPWNVL QCYLESTLQI GVIHEIIGNR AGAEGFLQLG
KAISCSQSLP LFIIVFSTVL GKLYHKQQLW DFAEKELQSA KQYLRASSTD ISCLKCRLML
EATVNQNLGD LFQSMFYNTR NTSLDKLSLA ENLYKSAIAK LNLSEWKNSV SCPEQGWVES
TRLRKTILKD VGSCASSTFT HSEENQEDIG KPTREGLKGK KEVKKCKKTN NAPKPVVKDQ
DAIPEYNLRS TRSRYQSSQN QSISGNGVVQ VGHSKQLKGN SKSDCPDTFR KREFLLDLKS
CEVAFGCDVT CICNKMSCWQ CLPVEVLDSG LVKNLVDLKW EFVRRRLSLR LLTGLGKCLE
SRGQIQETHE ITLQTVSILV SRNAFCLITS SVSSTSFLNL MGKEIPGDVF SVERAEVLLN
ISWLSLKSYC SKETRIICSD LPRIELPKLV SWLMLAFVLC RDVPVLFQKV SRLLAAIFLL
SASSERFSLS SSSKTLCENH WASYFHQASL GTHLSYQFFT NVSDICNVQH LVNAEVTGST
CMGSGKKKLL RLAPESIQEL EEFVTIFFAG LPCTTIICIS LLAGPYVSLL EELFPVPSCV
HAWILVSRLN SESQPIVMLL PVDSVLEGSA EDSDDVANSG SGSFSERKDT GKRWHCPWGF
TVVDKVAPEF RLILEESFSS ASLIFEEDTK NAWTFWWMWR QKLDLRLGKL LKNLEDSWFG
PWRCVLLGEW SNCKQLDLVH KKLVRDLKSK CKVDIDESLL KVILGGSKCA FEGGAYVSQL
CFKKGCYIGK AGCSGEEKCL TSPDESNGIE KESELAFQLI HEAVNELEGL CSVNREPIIL
VLDFEVQMLP WENLPILRNK EVYRMPSIGS ISSTLEKNYH HQDQVANNIT AFPLIDPLDS
FYLLNPSGDL GTTQIEFEKW FRDQNLEGKA GCAPPAEELA VALKSHDLFI YFGHGSGVQY
IPMHQIQRLE NCAATLLMGC SSGSLKLNGC YIPHGPPLSY LLAGSPVIVA NLWEVTDKDI
NRFAKAMLDG WLKERWSPSE GCVQCKVAEE FEAMSIRGRK GNAKKKISKK KLPEACESSD
PPIKISCDHK PKIGSFMSQA REACSLPFLT GASPVCYGVP TGIRKKDL
//
