ID A0A251S010_HELAN Unreviewed; 842 AA.
AC A0A251S010;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=HannXRQ_Chr16g0516591 {ECO:0000313|EMBL:OTF91954.1},
GN HanXRQr2_Chr16g0754891 {ECO:0000313|EMBL:KAF5760557.1};
OS Helianthus annuus (Common sunflower).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Heliantheae; Helianthus.
OX NCBI_TaxID=4232 {ECO:0000313|EMBL:OTF91954.1, ECO:0000313|Proteomes:UP000215914};
RN [1] {ECO:0000313|EMBL:KAF5760557.1, ECO:0000313|Proteomes:UP000215914}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. SF193 {ECO:0000313|Proteomes:UP000215914};
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5760557.1};
RX PubMed=28538728; DOI=10.1038/nature22380;
RA Badouin H., Gouzy J., Grassa C.J., Murat F., Staton S.E., Cottret L.,
RA Lelandais-Briere C., Owens G.L., Carrere S., Mayjonade B., Legrand L.,
RA Gill N., Kane N.C., Bowers J.E., Hubner S., Bellec A., Berard A.,
RA Berges H., Blanchet N., Boniface M.C., Brunel D., Catrice O., Chaidir N.,
RA Claudel C., Donnadieu C., Faraut T., Fievet G., Helmstetter N., King M.,
RA Knapp S.J., Lai Z., Le Paslier M.C., Lippi Y., Lorenzon L., Mandel J.R.,
RA Marage G., Marchand G., Marquand E., Bret-Mestries E., Morien E.,
RA Nambeesan S., Nguyen T., Pegot-Espagnet P., Pouilly N., Raftis F.,
RA Sallet E., Schiex T., Thomas J., Vandecasteele C., Vares D., Vear F.,
RA Vautrin S., Crespi M., Mangin B., Burke J.M., Salse J., Munos S.,
RA Vincourt P., Rieseberg L.H., Langlade N.B.;
RT "The sunflower genome provides insights into oil metabolism, flowering and
RT Asterid evolution.";
RL Nature 546:148-152(2017).
RN [2] {ECO:0000313|EMBL:OTF91954.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Leaves {ECO:0000313|EMBL:OTF91954.1};
RA Langlade N., Munos S.;
RT "Sunflower complete genome.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAF5760557.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5760557.1};
RA Gouzy J., Langlade N., Munos S.;
RT "Helianthus annuus Genome sequencing and assembly Release 2.";
RL Submitted (JUN-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MNCJ02000331; KAF5760557.1; -; Genomic_DNA.
DR EMBL; CM007905; OTF91954.1; -; Genomic_DNA.
DR STRING; 4232.A0A251S010; -.
DR EnsemblPlants; mRNA:HanXRQr2_Chr16g0754891; mRNA:HanXRQr2_Chr16g0754891; HanXRQr2_Chr16g0754891.
DR Gramene; mRNA:HanXRQr2_Chr16g0754891; mRNA:HanXRQr2_Chr16g0754891; HanXRQr2_Chr16g0754891.
DR InParanoid; A0A251S010; -.
DR OMA; CAHRANM; -.
DR OrthoDB; 5480520at2759; -.
DR Proteomes; UP000215914; Chromosome 16.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR019956; Ubiquitin_dom.
DR PANTHER; PTHR11254:SF424; E3 UBIQUITIN-PROTEIN LIGASE UPL5; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR PRINTS; PR00348; UBIQUITIN.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000215914};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 58..134
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000259|PROSITE:PS50053"
FT DOMAIN 502..842
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 808
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 842 AA; 96869 MW; 2B29F81C78BB71A0 CRC64;
MKRQLEDYDD DIFNELDLLR MRKFDPNPNS ASDTDHFLDQ IEASTSGSLQ HSSDPDRIHF
FVRMISNGKT LVLHGKPEDK VEVILEKIQS VTGIPMIEQR LIYQGRQLQS EYSLAECMIK
NDAGLHLVGR MRSTEHPQVW QLVDTLVSTI YRLLKGETEF ICKPVEAVLI VYLDMTAKRE
IRVNTLASHL NIFQSLCAPS ALVMLYLSPC KSNKELAEKS IRMFISACKT GLQRPLYSLC
APVALEFCNL LSRSVAQDDP LYSLCRSSLG SMVEDVRIGR SARNHDCFHN RMCMISFKDV
FPFVSELANK LAEGLDIDLL PSEVRDFAAF LRPIKAAIKD LPDFEGGIQL PMMQTLSCYD
DEVKDLSLIL FQLLEKMRRC LEKVKGEMEM KEKEDGDWDQ YLAILKETHG IAKLYQGGED
IFWSNMKVVD VSLRYLIVTY AKRGEDYKWV IGHKELLDFE SRRHLVMLML PETKSDDEEL
HEMLIDRSQL LSESFNYISK AKPENLRGGL FMEFNNEEAT GPGVLREWFF LVCQEIFNPQ
NALFIACPND RRRFFPNPAS KVDPLHLKYF KFAGRVTALA LMHKIQVGIM FDRTFFLQLA
GIDISLEDIK DADPYFYSSC KQILDMDPFI VDQDALGLTF VHEIEELGSM KVVELFTDGK
NIAVNSRNRK EYVELLIQHR FVTSVSKQVA KFAKGFADIV GDEKIQKLVF MTLEFEDHDG
LLHGSENIIS VDDWKAHTEY NGYTETDPQI YWFWEIVGEM TAEQRKVLLF FWTSLKHLPV
EGFSGLASKL YIYKSNDSID LLPSSHTCFY RICFPSYPSL EVMRQRLNII TQDHVGCGFG
TW
//
