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Database: UniProt
Entry: A0A251T5H2_HELAN
LinkDB: A0A251T5H2_HELAN
Original site: A0A251T5H2_HELAN 
ID   A0A251T5H2_HELAN        Unreviewed;       779 AA.
AC   A0A251T5H2;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   31-JUL-2019, entry version 11.
DE   SubName: Full=Putative urease {ECO:0000313|EMBL:OTG06407.1};
GN   Name=UREA {ECO:0000313|EMBL:OTG06407.1};
GN   ORFNames=HannXRQ_Chr12g0385371 {ECO:0000313|EMBL:OTG06407.1};
OS   Helianthus annuus (Common sunflower).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; asterids; campanulids; Asterales; Asteraceae;
OC   Asteroideae; Heliantheae alliance; Heliantheae; Helianthus.
OX   NCBI_TaxID=4232 {ECO:0000313|EMBL:OTG06407.1, ECO:0000313|Proteomes:UP000215914};
RN   [1] {ECO:0000313|Proteomes:UP000215914}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. SF193 {ECO:0000313|Proteomes:UP000215914};
RX   PubMed=28538728; DOI=10.1038/nature22380;
RA   Badouin H., Gouzy J., Grassa C.J., Murat F., Staton S.E., Cottret L.,
RA   Lelandais-Briere C., Owens G.L., Carrere S., Mayjonade B., Legrand L.,
RA   Gill N., Kane N.C., Bowers J.E., Hubner S., Bellec A., Berard A.,
RA   Berges H., Blanchet N., Boniface M.C., Brunel D., Catrice O.,
RA   Chaidir N., Claudel C., Donnadieu C., Faraut T., Fievet G.,
RA   Helmstetter N., King M., Knapp S.J., Lai Z., Le Paslier M.C.,
RA   Lippi Y., Lorenzon L., Mandel J.R., Marage G., Marchand G.,
RA   Marquand E., Bret-Mestries E., Morien E., Nambeesan S., Nguyen T.,
RA   Pegot-Espagnet P., Pouilly N., Raftis F., Sallet E., Schiex T.,
RA   Thomas J., Vandecasteele C., Vares D., Vear F., Vautrin S., Crespi M.,
RA   Mangin B., Burke J.M., Salse J., Munos S., Vincourt P.,
RA   Rieseberg L.H., Langlade N.B.;
RT   "The sunflower genome provides insights into oil metabolism, flowering
RT   and Asterid evolution.";
RL   Nature 546:148-152(2017).
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRSR:PIRSR611612-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRSR:PIRSR611612-51};
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR611612-50}.
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DR   EMBL; CM007901; OTG06407.1; -; Genomic_DNA.
DR   EnsemblPlants; OTG06407; OTG06407; HannXRQ_Chr12g0385371.
DR   Gramene; OTG06407; OTG06407; HannXRQ_Chr12g0385371.
DR   OMA; GFDSHIH; -.
DR   Proteomes; UP000215914; Chromosome 12.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:EnsemblPlants.
DR   GO; GO:0043419; P:urea catabolic process; IEA:InterPro.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000215914};
KW   Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR611612-51};
KW   Nickel {ECO:0000256|PIRSR:PIRSR611612-51};
KW   Reference proteome {ECO:0000313|Proteomes:UP000215914}.
FT   DOMAIN      399    779       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    590    590       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       404    404       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       406    406       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       487    487       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       487    487       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       516    516       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       542    542       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       630    630       Nickel 1. {ECO:0000256|PIRSR:PIRSR611612-
FT                                51}.
FT   BINDING     489    489       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     487    487       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR611612-50}.
SQ   SEQUENCE   779 AA;  83412 MW;  7F33E535F74D69E5 CRC64;
     MKLSPREVEK LMLHNAGFLA QKRLARAKRL NYPEAVALIA TQILEFVHDG DKSVAELMDI
     GRQLLGRRQV LPAVPHLLDS VQVEGTFPDG TKLITVHDPI SCENGNLDLA LQGSFLPVPS
     LEKFPDIEGG KIPGELILSN GNILLNAGRE AVKINVTNDG DRPIQVGSHY HFIEVNPSLI
     FDRRKSYGMR LNIPAGTATR FEPGDAKPVI LVRIGGNQVI RGGNAIADNH ANDSNVQTVM
     ESITARRFGN STDTGTSNGI IIEGSPLAYS MPREAYANMY GPTVGDKIRL GDTELFAQVE
     KDFAVYGDEC VFGGGKVIRD GMGQAAGFSA ADCLDTVITN ALIIDYTGIF KADIGIKGGC
     ISSLGKSGNP DAMNGVSDNM IIGVSTEVIA GEGMIVTAGA IDCHVHFICP QLAHEAISSG
     ITTVIGGGTG PADGTRATTC TPGPIHMKSM LQATDDLPLN FGFTGKGNSA KPEGIHEIIR
     AGAMGLKLHE DWGTTPATID NCLAVADQYD IQVNIHTDTL NESGFVEHTI AAFKDRTIHT
     YHSEGAGGGH APDIIKVCGV KNVLPSSTNP TRPFTKNTID EHLDMLMVCH HLDKNIPEDV
     AFAESRIRAE TIEAEDILHD MGAISIISSD SQAMGRIGEV ITRTWQTAHK MKLQRGPIDG
     NIPDNDNLRI KRYIAKYTIN PAIANGISDH VGSVEAGKLA DLVIWKPSFF GVKPEMVIKG
     GDIAWANMGD PNASIPTPEP VIMRPMFGAY GKAGSANSIA FVSKACCYGL QYKDLLWAQ
//
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