ID A0A251VCG7_HELAN Unreviewed; 1215 AA.
AC A0A251VCG7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:OTG32846.1};
GN Name=GCN2 {ECO:0000313|EMBL:OTG32846.1};
GN ORFNames=HannXRQ_Chr03g0091131 {ECO:0000313|EMBL:OTG32846.1},
GN HanXRQr2_Chr03g0134751 {ECO:0000313|EMBL:KAF5816481.1};
OS Helianthus annuus (Common sunflower).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Heliantheae; Helianthus.
OX NCBI_TaxID=4232 {ECO:0000313|EMBL:OTG32846.1, ECO:0000313|Proteomes:UP000215914};
RN [1] {ECO:0000313|EMBL:KAF5816481.1, ECO:0000313|Proteomes:UP000215914}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. SF193 {ECO:0000313|Proteomes:UP000215914};
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5816481.1};
RX PubMed=28538728; DOI=10.1038/nature22380;
RA Badouin H., Gouzy J., Grassa C.J., Murat F., Staton S.E., Cottret L.,
RA Lelandais-Briere C., Owens G.L., Carrere S., Mayjonade B., Legrand L.,
RA Gill N., Kane N.C., Bowers J.E., Hubner S., Bellec A., Berard A.,
RA Berges H., Blanchet N., Boniface M.C., Brunel D., Catrice O., Chaidir N.,
RA Claudel C., Donnadieu C., Faraut T., Fievet G., Helmstetter N., King M.,
RA Knapp S.J., Lai Z., Le Paslier M.C., Lippi Y., Lorenzon L., Mandel J.R.,
RA Marage G., Marchand G., Marquand E., Bret-Mestries E., Morien E.,
RA Nambeesan S., Nguyen T., Pegot-Espagnet P., Pouilly N., Raftis F.,
RA Sallet E., Schiex T., Thomas J., Vandecasteele C., Vares D., Vear F.,
RA Vautrin S., Crespi M., Mangin B., Burke J.M., Salse J., Munos S.,
RA Vincourt P., Rieseberg L.H., Langlade N.B.;
RT "The sunflower genome provides insights into oil metabolism, flowering and
RT Asterid evolution.";
RL Nature 546:148-152(2017).
RN [2] {ECO:0000313|EMBL:OTG32846.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Leaves {ECO:0000313|EMBL:OTG32846.1};
RA Langlade N., Munos S.;
RT "Sunflower complete genome.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAF5816481.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5816481.1};
RA Gouzy J., Langlade N., Munos S.;
RT "Helianthus annuus Genome sequencing and assembly Release 2.";
RL Submitted (JUN-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-
CC histidyl-tRNA(His); Xref=Rhea:RHEA:17313, Rhea:RHEA-COMP:9665,
CC Rhea:RHEA-COMP:9689, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57595, ChEBI:CHEBI:78442,
CC ChEBI:CHEBI:78527, ChEBI:CHEBI:456215; EC=6.1.1.21;
CC Evidence={ECO:0000256|ARBA:ARBA00001137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
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DR EMBL; MNCJ02000318; KAF5816481.1; -; Genomic_DNA.
DR EMBL; CM007892; OTG32846.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A251VCG7; -.
DR STRING; 4232.A0A251VCG7; -.
DR EnsemblPlants; mRNA:HanXRQr2_Chr03g0134751; mRNA:HanXRQr2_Chr03g0134751; HanXRQr2_Chr03g0134751.
DR Gramene; mRNA:HanXRQr2_Chr03g0134751; mRNA:HanXRQr2_Chr03g0134751; HanXRQr2_Chr03g0134751.
DR InParanoid; A0A251VCG7; -.
DR OMA; ADEAWND; -.
DR OrthoDB; 8734at2759; -.
DR Proteomes; UP000215914; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004694; F:eukaryotic translation initiation factor 2alpha kinase activity; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:EnsemblPlants.
DR GO; GO:0006521; P:regulation of cellular amino acid metabolic process; IEA:EnsemblPlants.
DR GO; GO:0009635; P:response to herbicide; IEA:EnsemblPlants.
DR CDD; cd14046; STKc_EIF2AK4_GCN2_rpt2; 1.
DR Gene3D; 3.40.50.800; Anticodon-binding domain; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004154; Anticodon-bd.
DR InterPro; IPR036621; Anticodon-bd_dom_sf.
DR InterPro; IPR041715; HisRS-like_core.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR006575; RWD_dom.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR PANTHER; PTHR11476; HISTIDYL-TRNA SYNTHETASE; 1.
DR PANTHER; PTHR11476:SF10; NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE; 1.
DR Pfam; PF03129; HGTP_anticodon; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF05773; RWD; 1.
DR Pfam; PF13393; tRNA-synt_His; 1.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF52954; Class II aaRS ABD-related; 1.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR PROSITE; PS50908; RWD; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000215914};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000313|EMBL:KAF5816481.1}.
FT DOMAIN 36..145
FT /note="RWD"
FT /evidence="ECO:0000259|PROSITE:PS50908"
FT DOMAIN 399..703
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..293
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..288
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 429
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 1215 AA; 136951 MW; FB39F3D77C7EDA21 CRC64;
MGHSSKKKKK RAGTGRRRPP SDHTSIDGVG GELLSDELTA LCAILQEDYD VVSESPPQIK
IKLRPYSQDS GYEDVDVSAF LSIRFLPGYP YKYPKLTIIP EKGLSKSDAD NLLSLLHDQA
NLNAREGRVM IYNLVEAAQE FLSEVVTIDQ PHLSRKNATV SCDRPSKGPF VYGFLDLFIG
SGESWHWGLS VEESNSTIVA LDNLNNGAQP SWEKVNPTLK MGVIEGGQLK VKSTERLDIL
EEDDSKSTDS LASQSAEPVE NVSVGEDYPL AEDTEEETDY DETESDILES ASSVDHHQIS
HTVERDLILA HLLRLACAPK GPLADALTDV TSELVNLGIV SDRVADLATG SSSLFDRSFN
QAFGNRMVAS KISNFWKTAA DSKGQHTSPT LSSRYLNDFE ELQPIGHGGF GHVVLCKNKL
DGRQYAVKKI RLKDKSQPLD DRILREVATL SRLQHQHVVR YYQAWFETGD AGFYDDATWG
SRTAASSSYS YIDHTSTDVI GQDNKVGTYL YIQMEYCPRT LRQMFESYSH FDKKDAWHLF
RQIVEGLAHI HSQGIIHRDL TPNNIFFDAR SDIKIGDFGL AKFLKLEQLD QDVDPAETTG
VSVDGTGQIG TYFYTAPEIE QRWPKINEKA DMYSLGIVFF ELWHPFSTGM ERHIVLSDLK
KKGELPSDWV AEFPEQAALL RRMMSSSPSD RPSANELLQH ACPPRMESEL LDNILRTMHN
SEDTSIYDKV VNAIFSEEIL STKIQDNDTE TLKLGRNVTS SIQHTDLDTE IRDLVSEVSA
AVFRLHCAKH LETIPMRLLG DSVQFNRNNV KLLTNGGDML ELCHELRLPF INWVVLNQKS
TFKRFEIGYV YRRSIGHSPP NRYLQGDFDV IGGDSAITEA EVIKATMDII TRFFHPESCD
IHLNHGDLLE AIWSWIGIKA DHRQKVAELL SLLGSLRPQS TERKTKWVVI RRQLRQELNI
TEGAVNKLQT VGLRFCGSAD HALPRLRGAL PSDTLTRKAL DELSQLFNYL RIWKIDKHVF
IDPLMPPTEG YHRDSFFQIY LRKDNTLGSH TEGTLLAVGG RYDYLLHRMW QSEYKSNPPG
AVGTSLALET IIHHSSVDVY RPFRNDSSRS VLVCSRGGGG LLEKRMELVT ELREDDIKAE
FVPTLDPSLT EQYEYANEND IKCLIIISDT GVSQNGSVKI RHLELKKEKE VSRESLANFL
SDAMASQFRN PSIWN
//
