ID A0A251WGH8_9CYAN Unreviewed; 603 AA.
AC A0A251WGH8;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311};
GN ORFNames=B0A82_11450 {ECO:0000313|EMBL:OUC14545.1};
OS Alkalinema sp. CACIAM 70d.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Leptolyngbyales; Leptolyngbyaceae;
OC Alkalinema.
OX NCBI_TaxID=1934309 {ECO:0000313|EMBL:OUC14545.1, ECO:0000313|Proteomes:UP000194922};
RN [1] {ECO:0000313|EMBL:OUC14545.1, ECO:0000313|Proteomes:UP000194922}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CACIAM 70d {ECO:0000313|EMBL:OUC14545.1};
RA Lima A.R., Siqueira A.S., Pereira J.S., Goncalves E.C.;
RT "Draft genome of Alkalinema sp. CACIAM 70d.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUC14545.1}.
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DR EMBL; MUGG01000104; OUC14545.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A251WGH8; -.
DR STRING; 1934309.B0A82_11450; -.
DR Proteomes; UP000194922; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR CDD; cd08639; DNA_pol_A_Aquificae_like; 1.
DR CDD; cd06142; RNaseD_exo; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA-directed DNA polymerase {ECO:0000313|EMBL:OUC14545.1};
KW Nucleotidyltransferase {ECO:0000313|EMBL:OUC14545.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000194922};
KW Transferase {ECO:0000313|EMBL:OUC14545.1}.
FT DOMAIN 15..186
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 361..563
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 603 AA; 67376 MW; DFECCE48E28C9695 CRC64;
MLVSKTYPNL KDVTYHLITS TKELEIALRS LEQSSILGLD CETTGLDPHT HTLRLIQIAV
PNQPVILIDL PQIAPCDRGL LQQFLAKPTL KITHNGKFDW QFLTQAGLQP SAPFFDTQLA
YRVLTAGLKT SLSLQTLAQK LLNIELDKTQ QVSDWSKPLT SQQLQYAALD AAILLELYPI
LVKKLERWQS LPAGNLQLLK IAQLEFHCMP AVAQMELNGM LLDLSRWQRL GAKLESDRDT
ALQQLSQLRI AGNAQMSLLP EMTDTINPNS PQQVLAALQS SEIPVSSTHQ KELVPLAKHY
PVIRALLDYR HLSKIMATFA DSLPKHIHPV TGRIHPTYYQ LGARSGRFSC RNPPLQTIPR
DAAVRTCFIA APGCQMVRAD YSQIELRIVA RLSGDARMQR AYRKGEDLHR LTAALVTGKA
IADVSEEDRR LAKAINFGLI YGMGATKLQS YAETKYGVTL SLEQAKAFRK RFFEAYAGVA
EWHETIKRNY IRGIKESRTL AGRRRRWADK PRLAELLNHP VQGLNADITK LALVKLNKVL
VGTGAKLICT VHDEILLECP AAEAKHISYL LLRCMIAAAR KFLHPIPVVV DIKVSASWGG
DET
//
