ID A0A252BMG6_9PROT Unreviewed; 289 AA.
AC A0A252BMG6;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
GN ORFNames=HK24_04420 {ECO:0000313|EMBL:OUJ08018.1};
OS Gluconobacter sp. DsW_058.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Gluconobacter.
OX NCBI_TaxID=1511210 {ECO:0000313|EMBL:OUJ08018.1, ECO:0000313|Proteomes:UP000194786};
RN [1] {ECO:0000313|Proteomes:UP000194786}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Winans N.J., Newell P.D., Douglas A.E.;
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|RuleBase:RU003862};
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway. {ECO:0000256|ARBA:ARBA00034478}.
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUJ08018.1}.
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DR EMBL; JOPH01000034; OUJ08018.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A252BMG6; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000194786; Unassembled WGS sequence.
DR GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003862};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003862};
KW Reference proteome {ECO:0000313|Proteomes:UP000194786}.
SQ SEQUENCE 289 AA; 32083 MW; 2EFEEB05C39FD796 CRC64;
MTISVGQVRA DLMKESSIEL LPSRILELSG VLPDLACGSR VFVTWLPGVP FADVLAACDL
LKLCGYLPVP HVAARSIRDE GHLQEICSAL TERDIPRMLL IAGGADKPAG IFADTVQILE
TEIPVRTGIR TVYVAGHPEG HPQTSEATLL EYMRRKQELA RTQGLNMQIV TQFCFDSAPV
LAWEKRLRDG GITLPINLGV YGLTTPKTLV RYGLACGVGQ SLRFMQRQRW KLHRWFMPCN
PQHLVDAVAQ EIAQTPDTNF RGLHFYPFGA IARTLEWRRA LSLSTGDHS
//
